Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GCKR.
(Peptide available as ab97365.)
- This antibody gave a positive signal in Human liver tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Inhibits glucokinase by forming an inactive complex with this enzyme.
Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
Belongs to the GCKR family.
Contains 2 SIS domains.
Information by UniProt
- FGQTL5 antibody
- GCK antibody
- GCKR antibody
Western blot - GCKR antibody (ab87739)
Anti-GCKR antibody (ab87739) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889
) at 10 µgSecondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
68 kDaObserved band size:
68 kDaAdditional bands at:
29 kDa. We are unsure as to the identity of these extra bands.Exposure time:
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"