Anti-GCDH/GCD抗体[3E9BA3BF5] (ab112998)
Key features and details
- Mouse monoclonal [3E9BA3BF5] to GCDH/GCD
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-GCDH/GCD抗体[3E9BA3BF5]
参阅全部 GCDH/GCD 一抗 -
描述
小鼠单克隆抗体[3E9BA3BF5] to GCDH/GCD -
宿主
Mouse -
经测试应用
适用于: ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Fibroblast, HeLa, HepG2, and Rat Cardiomyocyte cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
ab112998 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
克隆
单克隆 -
克隆编号
3E9BA3BF5 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112998于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
0.1% Triton X-100 permeabilization recommended. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. 0.1% Triton X-100 permeabilization recommended. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive. -
组织特异性
Isoform 1 and isoform 2 are expressed in fibroblasts and liver. -
通路
Amino-acid metabolism; lysine degradation.
Amino-acid metabolism; tryptophan metabolism. -
疾病相关
Defects in GCDH are the cause of glutaric aciduria type 1 (GA1) [MIM:231670]. GA1 is an autosomal recessive metabolic disorder characterized by progressive dystonia and athetosis due to gliosis and neuronal loss in the basal ganglia. -
序列相似性
Belongs to the acyl-CoA dehydrogenase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 2639 Human
- Omim: 608801 Human
- SwissProt: Q92947 Human
- Unigene: 532699 Human
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别名
- ACAD5 antibody
- EC 1.3.99.7 antibody
- GCD antibody
see all
图片
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ab112998 at 1 µg/ml staining GCDH/GCD in fibroblast cells by immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI.
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ab112998 at 1ug/ml staining GCDH/GCD in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab112998 尚未被引用在任何文献中。