Anti-GCAP2抗体[A1] (ab5422)

概述

  • 产品名称
    Anti-GCAP2抗体[A1]
    参阅全部 GCAP2 一抗
  • 描述
    小鼠单克隆抗体[A1] to GCAP2
  • 特异性
    This antibody is specific for GCAP 2 and does not react with other isotypes.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Cow
  • 免疫原

    Other Immunogen Type corresponding to GCAP2. Bacterial expressed full length GCAP2.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度
    Protein A purified
  • Primary antibody说明
    Guanylate cyclase-activating proteins (GCAPs) are calcium binding proteins that belong to the calmodulin superfamily. GCAPs without calcium are responsible for activation of photoreceptor guanylate cyclase during light adaptation. Studies have shown that the addition or subtraction of Ca2+ results in major conformational changes and the activation/deactivation of GCAP 1 and GCAP 2. It has been demonstrated that both GCAP 1 and 2 act on guanylate cyclase similarly and have approximately 50% homology between the two forms. GCAP-1 is predominantly localized in the photoreceptor outer segments, while GCAP 2 is found in retina samples.
  • 克隆
    单克隆
  • 克隆编号
    A1
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa.

靶标

  • 功能
    Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low, and GC1 and GC2 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.
  • 组织特异性
    Retina. Cones and rod.
  • 序列相似性
    Contains 4 EF-hand domains.
  • 细胞定位
    Cell membrane. Membranes of outer segment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GCAP 2 antibody
    • Guanylate Cyclase Activating Protein Photoreceptor 2 antibody
    • Guanylate cyclase activator 1B antibody
    • Guanylin 2 antibody
    • Guanylyl cyclase-activating protein 2 antibody
    • GUC1B_HUMAN antibody
    • GUCA 2 antibody
    • Guca1b antibody
    see all

实验方案

文献

ab5422 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry. Both of these antibodies have been made against the full length GCAP1 and full length GCAP2 proteins. These antibodies have not been epitope mapped, and therefore we do not know the exact peptide sequence that the anti-GCAP...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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