Anti-GC1q R抗体[60.11] (ab24733)

概述

  • 产品名称Anti-GC1q R抗体[60.11]
    参阅全部 GC1q R 一抗
  • 描述
    小鼠单克隆抗体[60.11] to GC1q R
  • 特异性This antibody recognizes the mature receptor at the amino terminus (aa 76-93), in the region of the receptor that binds C1q. It will block the C1q/gC1q-R interaction.
  • 经测试应用适用于: Neutralising, IHC-P, WB, IP, ELISA, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Bacterial expressed recombinant full length protein GC1q R.

  • 阳性对照
    • This antibody gave a positive signal in Human tonsil tissue sections. This antibody gave a positive signal in the following Methanol fixed cell lines: HeLa.
  • 常规说明

    Alternative versions available:

    Anti-GC1q R antibody [60.11] - BSA and Azide free (ab176244)

    Anti-GC1q R antibody (HRP) [60.11] (ab199987)

     

     

     

性能

应用

Our Abpromise guarantee covers the use of ab24733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Neutralising Use at an assay dependent concentration. PubMed: 9233640
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP 1/5000.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Binds to the globular "heads" of C1Q thus inhibiting C1 activation.
  • 序列相似性Belongs to the MAM33 family.
  • 细胞定位Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASF/SF2 associated protein p32 antibody
    • C1q globular domain binding protein antibody
    • C1qBP antibody
    • C1QBP_HUMAN antibody
    • Complement component 1 q subcomponent binding protein antibody
    • Complement component 1 Q subcomponent binding protein mitochondrial antibody
    • Complement component 1 Q subcomponent-binding protein, mitochondrial antibody
    • GC1Q R antibody
    • GC1q R protein antibody
    • GC1q-R protein antibody
    • GC1QBP antibody
    • GC1QR antibody
    • globular domain of, C1q, receptor for antibody
    • Glycoprotein gC1qBP antibody
    • HABP 1 antibody
    • HABP1 antibody
    • Hyaluronan binding protein 1 antibody
    • Hyaluronan-binding protein 1 antibody
    • Mitochondrial matrix protein p32 antibody
    • p32 antibody
    • p32 splicing factor antibody
    • p33 antibody
    • Pre mrna splicing factor SF2 P32 subunit precursor antibody
    • SF2p32 antibody
    • Splicing factor SF2 associated protein antibody
    see all

Anti-GC1q R antibody [60.11] 图像

  • All lanes : Anti-GC1q R antibody [60.11] (ab24733) at 5 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Lane 1 : Mw standards
    Lanes 2 - 3 : Anti-GC1q R antibody [60.11] (ab24733) at 1/2000 dilution

    Lane 1 : As above
    Lane 2 : 293T whole cell lysate at 20 µg
    Lane 3 : Hela whole cell lysate at 20 µg

    Secondary
    Lanes 2 - 3 : Alexa Fluor® 680 conjugated goat anti-mouse

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    The blot was blocked with 5% milk for 1 hour at 25°C prior to incubating with the primary antibody for 13 hours at 4°C.

    See Abreview

  • ab24733 used in Flow Cytometry.
    U937 cells were cultured in RPMI 10% FCS, treated with PMA (5nM) and, 6-12 hours later, cells were harvested, spinned and resuspended to 200,000 cells in 100µl. ab24733 used at 10µg/ml for 30 minutes at 4°C. A phycoerythrin conjugated goat anti-mouse polyclonal was used as the secondary antibody at a 1/100 dilution.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab24733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab24733, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150117) at 1/4000 dilution for 30 min at 22°C.

    Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (ab170190, 0.5μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • IHC image of ab24733 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24733, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab24733 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24733 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GC1q R antibody [60.11] (ab24733)参考文献

This product has been referenced in:
  • Westman J  et al. p33 (gC1q receptor) prevents cell damage by blocking the cytolytic activity of antimicrobial peptides. J Immunol 191:5714-21 (2013). Read more (PubMed: 24174616) »
  • Sanchez-Martin D  et al. The multi-compartmental p32/gClqR as a new target for antibody-based tumor targeting strategies. J Biol Chem : (2010). Flow Cyt . Read more (PubMed: 21156793) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (gradient 4% to 12%)
Sample Pig Cell lysate - whole cell (PK15)
Specification PK15
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Mr. Guy Kouokam

Verified customer

提交于 Jan 23 2015

Thank you for your reply.

I will recommend you to use the next antibody as an isotype control for Functional Assays:

http://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MG1-45-Isotype-control-ab18447.html

As it is suitable fo...

Read More

Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

Es tut mir leid zu hören, dass unsere Vorschläge in diesem Fall nicht zu einer Verbesserung Ihrer Ergebnisse geführt haben. Ich weiß, dass Sie viel Zeit für diese Experimente...

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Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

Um Ihr Problem zu lösen, möchte ich die folgenden Vor...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Human Cell (U-937 (ATCC: CRL-1593.2) histiocytic l)
Specification Human Cell (U-937 (ATCC: CRL-1593.2) histiocytic l
Preparation Cell harvesting/tissue preparation method: U937 cells were culture in RPMI 10% FCS, treated with PMA (5nM) and, 6-12 hours later, cells were harvested, spinned and resuspend to 200 000cells in 100ul
Sample buffer: PBS + 0.1% FCS
Fixation No fixation
Permeabilization No
Gating Strategy FS - SS
Username

Mr. David Sanchez Martin

Verified customer

提交于 Jan 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela and 293T)
Loading amount 20 µg
Specification Hela and 293T
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 23 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"