Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Pig, African green monkey
Synthetic peptide: KSHTKGIWMW CVPHPKKPEH TLVLLDTEGL GDIEKGDNEN DSWIFALAIL, corresponding to a region within N terminal amino acids 72 - 121 of Human GBP2 (NP_004111).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Guanylate-binding proteins (GBPs) are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). GBP2 is a GTPase that converts GTP to GDP and GMP.
Cell membrane; Lipid-anchor; Cytoplasmic side
- GBP 2 antibody
- GTP binding protein 2 antibody
- Guanine nucleotide binding protein 2 antibody
Western blot - GBP2 antibody (ab81486)
Anti-GBP2 antibody (ab81486) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
67 kDaObserved band size:
65 kDa (why is the actual band size different from the predicted?
)Additional bands at:
30 kDa, 32 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"