Synthetic peptide derived from an internal sequence of human GBP1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Predicted molecular weight: 68 kDa.
||1/500 - 1/1000.
Anti-GBP1 antibody 图像
Western blot - GBP1 antibody (ab74864)
All lanes :
Anti-GBP1 antibody (ab74864) at 1/500 dilutionLane 1 :
Extracts from HUVEC cellsLane 2 :
Extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.Predicted band size :
68 kDaObserved band size :
75 kDa (why is the actual band size different from the predicted?
)Additional bands at :
83 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - GBP1 antibody (ab74864)
Immunofluorescence analysis of GBP1 in MCF-7 cells, using
ab74864, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Anti-GBP1 antibody (ab74864)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"