Anti-GBP1抗体[OTI1B2] (ab119236)
Key features and details
- Mouse monoclonal [OTI1B2] to GBP1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-GBP1抗体[OTI1B2]
参阅全部 GBP1 一抗 -
描述
小鼠单克隆抗体[OTI1B2] to GBP1 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human GBP1 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_002044).
Database link: P32455 -
阳性对照
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY GBP1 cDNA, Mouse spleen extract; Human testis, omentum, uterus, liver, ovary, thyroid gland, colon and spleen extracts. IHC-P: Human lung carcinoma tissue. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY GBP1 cDNA. Flow Cyt (Intracellular): HEK-293T cells transfected with pCMV6-ENTRY GBP1 cDNA.
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常规说明
Clone OTI1B2 (formerly 1B2).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant -
克隆
单克隆 -
克隆编号
OTI1B2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119236于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/100.
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WB |
1/2000. Predicted molecular weight: 68 kDa.
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IHC-P |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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说明 |
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Flow Cyt (Intra)
1/100. |
WB
1/2000. Predicted molecular weight: 68 kDa. |
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
靶标
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功能
Binds GTP, GDP and GMP. -
序列相似性
Belongs to the GBP family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2633 Human
- Omim: 600411 Human
- SwissProt: P32455 Human
- SwissProt: Q01514 Mouse
- Unigene: 62661 Human
- Unigene: 457978 Mouse
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别名
- GBP 1 antibody
- GBP-1 antibody
- GBP1 antibody
see all
图片
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All lanes : Anti-GBP1 antibody [OTI1B2] (ab119236) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY GBP1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 68 kDa -
Paraffin-embedded human lung carcinoma tissue stained for GBP1 using ab119236 at 1/150 in immunohistochemical analysis.
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COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY GBP1 cDNA, stained for GBP1 using ab119236 at 1/100 in ICC/IF.
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All lanes : Anti-GBP1 antibody [OTI1B2] (ab119236) at 1/200 dilution
Lane 1 : Human testis extract
Lane 2 : Human omentum extract
Lane 3 : Human uterus extract
Lane 4 : Human breast extract
Lane 5 : Human brain extract
Lane 6 : Human liver extract
Lane 7 : Human ovary extract
Lane 8 : Human thyroid gland extract
Lane 9 : Human colon extract
Lane 10 : Human spleen extract
Lysates/proteins at 10 µg per lane.
Predicted band size: 68 kDa -
Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY GBP1 (red) or empty vector control plasmid (blue) labeling GBP1 with ab119236 at 1/100 dilution.
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Anti-GBP1 antibody [OTI1B2] (ab119236) at 1/200 dilution + Mouse spleen extract at 10 µg
Predicted band size: 68 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab119236 被引用在 1 文献中.
- Pesciotta EN et al. In-Depth, Label-Free Analysis of the Erythrocyte Cytoplasmic Proteome in Diamond Blackfan Anemia Identifies a Unique Inflammatory Signature. PLoS One 10:e0140036 (2015). PubMed: 26474164