The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer. May play an important role in the transcriptional program(s) that underlies smooth muscle cell diversity.
Immunocytochemistry/ Immunofluorescence - Anti-GATA5 antibody (ab48820)Image courtesy of an anonymous Abreview.
ab48820 staining GATA5 in murine epicardial explant culture by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permeabilized using 0.5% Triton X-100 in PBS, blocked with 1% goat serum 10% BSA 0.1% Triton X-100 PBS and then incubated with ab48820 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit IgG (H+L) used at a 1/200 dilution.