概述

  • 产品名称Anti-GATA5抗体
    参阅全部 GATA5 一抗
  • 描述
    兔多克隆抗体to GATA5
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide: SGCEPSPQPP ELAARPGWAQ TATADSSAFG PGSPHPPAAH PPGATAFPFA, corresponding to N terminal amino acids 47-96 of Human GATA5

  • 阳性对照
    • HepG2 cell lysate; Human skeletal muscle tissue.

性能

应用

Our Abpromise guarantee covers the use of ab118777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-GATA5 antibody 图像

  • Anti-GATA5 antibody (ab118777) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 25 kDa
  • Immunohistochemistry (Formalin-PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling GATA5 with ab118777 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

Anti-GATA5 antibody (ab118777)参考文献

ab118777 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"