Anti-GATA4 (phospho S105)抗体(ab5245)

概述

  • 产品名称
    Anti-GATA4 (phospho S105)抗体
    参阅全部 GATA4 一抗
  • 描述
    兔多克隆抗体to GATA4 (phospho S105)
  • 经测试应用
    适用于: ICC/IF, EMSA, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog
    预测可用于: Chicken, Human
  • 免疫原

    Synthetic peptide (Human) derived from a region of GATA 4 that contains serine 105.

  • 阳性对照
    • Phenylephrine (PE)-stimulated rat cardiomyocytes, transfected with mouse GATA 4.

性能

应用

Our Abpromise guarantee covers the use of ab5245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 19546173
EMSA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 22919071
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).

靶标

  • 功能
    Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • 疾病相关
    Atrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 序列相似性
    Contains 2 GATA-type zinc fingers.
  • 翻译后修饰
    Methylation at Lys-300 attenuates transcriptional activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Anti-GATA4 (phospho S105) antibody 图像



  • Predicted band size : 46 kDa


    Extracts prepared from cardiomyocytes overexpressing wild-type GATA-4 (1-4 from left to right) stimulated with phenylephrine (PE) for 24 hours were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.35 µg/mL ab5245 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to GATA-4 [pS105] blocks the antibody signal, and that the S105A mutant does not react, thereby demonstrating the specificity of the antibody.

Anti-GATA4 (phospho S105) antibody (ab5245)参考文献

This product has been referenced in:
  • Tae HJ  et al. G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing ß1- and ß2-adrenergic receptors. Mol Med Rep 15:4049-4054 (2017). Read more (PubMed: 28487987) »
  • Tasoglu S  et al. Guided and magnetic self-assembly of tunable magnetoceptive gels. Nat Commun 5:4702 (2014). ICC/IF ; Rat . Read more (PubMed: 25175148) »

See all 10 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Vascular smooth muscle cell)
Permeabilization
Yes - NP40
Specification
Vascular smooth muscle cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 04 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (pancreatic cancer)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
pancreatic cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 09 2016

Thank you for your protocol details, I'm very sorry to hear you are experiencing problems with ab5245 and would like to recommend the following advice: 1) please make sure that the protein is in its phosphorylated form: do you have a stimulation pa...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册