概述

  • 产品名称Anti-GATA4抗体
    参阅全部 GATA4 一抗
  • 描述
    兔多克隆抗体to GATA4
  • 特异性Detects total GATA4 levels.
  • 经测试应用适用于: ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from human GATA4 around the phosphorylation site of serine 262 (R-L-SP-A-S).

  • 阳性对照
    • HuvEc cells.

性能

应用

Our Abpromise guarantee covers the use of ab61170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • 疾病相关Atrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 序列相似性Contains 2 GATA-type zinc fingers.
  • 翻译后修饰Methylation at Lys-300 attenuates transcriptional activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Anti-GATA4 antibody 图像

  • 1/500-1/1000 ab61170 staining GATA4 on HeLa cells, in the absence or presence of the immunizing peptide.

Anti-GATA4 antibody (ab61170)参考文献

This product has been referenced in:
  • Oltolina F  et al. Human Cardiac Progenitor Spheroids Exhibit Enhanced Engraftment Potential. PLoS One 10:e0137999 (2015). ICC/IF ; Human . Read more (PubMed: 26375957) »
  • Huang WC  et al. Treatment of Glucocorticoids Inhibited Early Immune Responses and Impaired Cardiac Repair in Adult Zebrafish. PLoS One 8:e66613 (2013). ICC/IF . Read more (PubMed: 23805247) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (differentiated iPSC)
Specification differentiated iPSC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Apr 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"