Anti-GATA4抗体(ab124265)

概述

  • 产品名称Anti-GATA4抗体
    参阅全部 GATA4 一抗
  • 描述
    兔多克隆抗体to GATA4
  • 经测试应用适用于: IHC-P, ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 392-442 of Human GATA4 (NP_002043.2).

  • 阳性对照
    • 293T whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab124265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB 1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
IP Use a concentration of 2 - 10 µg/ml.

靶标

  • 功能Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • 疾病相关Atrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 序列相似性Contains 2 GATA-type zinc fingers.
  • 翻译后修饰Methylation at Lys-300 attenuates transcriptional activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Anti-GATA4 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach linitis plastica tissue labelling GATA4 with ab124265 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-GATA4 antibody (ab124265) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg/ml
    Lane 2 : 293T whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 50 µg/ml
    Lane 4 : Jurkat whole cell lysate at 50 µg/ml

    Developed using the ECL technique

    Predicted band size : 44 kDa


    Exposure time : 3 minutes
  • ab124265 at 1 µg/ml detecting GATA4 in 293T whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes an upstream epitope of GATA4
    Lane 2: ab124265 at 6 µg/mg of lysate
    Lane 3: Control IgG

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 10 seconds
  • ab124265 staining GATA4 in human pluripotent stem cell derived cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/800) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.

    Nuclear specific staining of GATA4 (yellow) in GFP positive cardiomyocytes (green), with GFP driven by a cardiac specific promoter.

    See Abreview

Anti-GATA4 antibody (ab124265)参考文献

ab124265 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde
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提交于 Sep 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"