GATA3 overexpression 293T裂解液(全细胞) (ab94325)

概述

  • 产品名称GATA3 overexpression 293T裂解液(全细胞)
  • 描述
    GATA3 overexpression裂解液
  • 常规说明ab94325 is a 293T cell transfected lysate in which Human GATA3 has been transiently over-expressed using a pCMV-GATA3 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Disease: Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome. Function: Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Similarity: Contains 2 GATA-type zinc fingers. Tissue specificity: T-cells and endothelial cells.

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应用

Our Abpromise guarantee covers the use of ab94325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

GATA3 overexpression 293T lysate (whole cell) 图像

  • ab94325 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-GATA3 antibody (ab89776) at 1/500 dilution

    Lane 1 : GATA3 overexpression 293T lysate (whole cell) (ab94325)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

GATA3 overexpression 293T lysate (whole cell) (ab94325)参考文献

ab94325 has not yet been referenced specifically in any publications.

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