Anti-GATA3抗体[mAbcam77073] (ab77073)

概述

  • 产品名称Anti-GATA3抗体[mAbcam77073]
    参阅全部 GATA3 一抗
  • 描述
    小鼠单克隆抗体[mAbcam77073] to GATA3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human GATA3.

    (Peptide available as ab107920.)

  • 阳性对照
    • This antibody gave a positive signal in the following human tissue lysates: Thymus; Lymph Node; Spleen; Tonsil.

性能

应用

Our Abpromise guarantee covers the use of ab77073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

靶标

Anti-GATA3 antibody [mAbcam77073] 图像

  • All lanes : Anti-GATA3 antibody [mAbcam77073] (ab77073) at 5 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Tonsil (Human) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-GATA3 antibody [mAbcam77073] (ab77073)参考文献

ab77073 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77073.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"