Anti-GATA3抗体[mAbcam77073] (ab77073)

概述

  • 产品名称
    Anti-GATA3抗体[mAbcam77073]
    参阅全部 GATA3 一抗
  • 描述
    小鼠单克隆抗体[mAbcam77073] to GATA3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human GATA3.

    (Peptide available as ab107920.)

  • 阳性对照
    • This antibody gave a positive signal in the following human tissue lysates: Thymus; Lymph Node; Spleen; Tonsil.
  • 常规说明

    This antibody clone is manufactured by Abcam.

性能

应用

Our Abpromise guarantee covers the use of ab77073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

靶标

图片

  • All lanes : Anti-GATA3 antibody [mAbcam77073] (ab77073) at 5 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Tonsil (Human) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

实验方案

文献

ab77073 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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