Anti-GATA3抗体[mAbcam77073] (ab77073)

概述

  • 产品名称
    Anti-GATA3抗体[mAbcam77073]
    参阅全部 GATA3 一抗
  • 描述
    小鼠单克隆抗体[mAbcam77073] to GATA3
  • 宿主
    Mouse
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human GATA3.

    (Peptide available as ab107920.)

  • 阳性对照
    • This antibody gave a positive signal in the following human tissue lysates: Thymus; Lymph Node; Spleen; Tonsil.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

应用

Our Abpromise guarantee covers the use of ab77073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

靶标

图片

  • All lanes : Anti-GATA3 antibody [mAbcam77073] (ab77073) at 5 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Tonsil (Human) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    The binding pattern of ab77073 in human thymus, lymph node, spleen and tonsil tissue lysates is similar to that of the polyclonal antibody to the same target (ab32858) in MOLT4, Jurkat, THP1 and Ramos whole cell lysates.

实验方案

文献

ab77073 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab77073.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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