Anti-GATA3抗体(ab82828)

概述

  • 产品名称Anti-GATA3抗体
    参阅全部 GATA3 一抗
  • 描述
    兔多克隆抗体to GATA3
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    3 synthetic peptides (human) conjugated to KLH

  • 阳性对照
    • Recombinant GATA3 protein expressed in induced culture of E.coli (one shot Top10 competent cells).

性能

应用

Our Abpromise guarantee covers the use of ab82828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at an assay dependent dilution. Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
    WB: 1/1000. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
    • 组织特异性T-cells and endothelial cells.
    • 疾病相关Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
    • 序列相似性Contains 2 GATA-type zinc fingers.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 形式There are 2 isoforms produced by alternative splicing.
    • 别名
      • GATA 3 antibody
      • GATA binding factor 3 antibody
      • GATA binding protein 3 antibody
      • GATA-binding factor 3 antibody
      • Gata3 antibody
      • GATA3_HUMAN antibody
      • HDR antibody
      • HDRS antibody
      • MGC2346 antibody
      • MGC5199 antibody
      • MGC5445 antibody
      • Trans acting T cell specific transcription factor GATA 3 antibody
      • Trans-acting T-cell-specific transcription factor GATA-3 antibody
      see all

    Anti-GATA3 antibody 图像

    • All lanes : Anti-GATA3 antibody (ab82828) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)

    Anti-GATA3 antibody (ab82828)参考文献

    ab82828 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. I have checked our catalog and found 4 anti-GATA3 antibodies that have been tested in mouse (ab32858, ab106625, ab82828 and ab110093), however none of these antibodies has been tested so far in frozen tissue section...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"