Anti-GATA3抗体(ab82828)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to GATA3
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    3 synthetic peptides (human) conjugated to KLH

  • 阳性对照
    • Recombinant GATA3 protein expressed in induced culture of E.coli (one shot Top10 competent cells).

性能

应用

Our Abpromise guarantee covers the use of ab82828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: Use at an assay dependent dilution. Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
    WB: 1/1000. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
    • 组织特异性
      T-cells and endothelial cells.
    • 疾病相关
      Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
    • 序列相似性
      Contains 2 GATA-type zinc fingers.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 形式
      There are 2 isoforms produced by alternative splicing.
    • 别名
      • GATA 3 antibody
      • GATA binding factor 3 antibody
      • GATA binding protein 3 antibody
      • GATA-binding factor 3 antibody
      • Gata3 antibody
      • GATA3_HUMAN antibody
      • HDR antibody
      • HDRS antibody
      • MGC2346 antibody
      • MGC5199 antibody
      • MGC5445 antibody
      • Trans acting T cell specific transcription factor GATA 3 antibody
      • Trans-acting T-cell-specific transcription factor GATA-3 antibody
      see all

    图片

    • All lanes : Anti-GATA3 antibody (ab82828) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    ab82828 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Thank you for contacting us. I have checked our catalog and found 4 anti-GATA3 antibodies that have been tested in mouse (ab32858, ab106625, ab82828 and ab110093), however none of these antibodies has been tested so far in frozen tissue section...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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