概述

  • 产品名称
  • 描述
    山羊多克隆抗体to GATA3
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    EVTADQPRWVSHH

    conjugated to KLH, corresponding to N terminal amino acids 2-14 of Human GATA3 (CAL37566.1).

  • 阳性对照
    • Human Heart and Human Placenta tissues, MOLT-4 lysate

性能

应用

Our Abpromise guarantee covers the use of ab113519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • 组织特异性
    T-cells and endothelial cells.
  • 疾病相关
    Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • 序列相似性
    Contains 2 GATA-type zinc fingers.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 形式
    There are 2 isoforms produced by alternative splicing.
  • 别名
    • GATA 3 antibody
    • GATA binding factor 3 antibody
    • GATA binding protein 3 antibody
    • GATA-binding factor 3 antibody
    • Gata3 antibody
    • GATA3_HUMAN antibody
    • HDR antibody
    • HDRS antibody
    • MGC2346 antibody
    • MGC5199 antibody
    • MGC5445 antibody
    • Trans acting T cell specific transcription factor GATA 3 antibody
    • Trans-acting T-cell-specific transcription factor GATA-3 antibody
    see all

图片

  • Anti-GATA3 antibody (ab113519) at 3 µg/ml + MOLT-4 lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 48 kDa
  • Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab113519 at a concentration of 3.75 µg/ml.
  • Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab113519 at a concentration of 3.75 µg/ml.

文献

This product has been referenced in:
  • Gu W  et al. Effects of Shen-Fu Injection on the Expression of T-Cell-Specific Transcription Factors T-bet/Gata-3 in Porcine Postresuscitation Lung Injury. Evid Based Complement Alternat Med 2013:464650 (2013). WB . Read more (PubMed: 23573129) »

See 1 Publication for this product

客户评价及客户问答

I am sorry to see that the Abcam anti-GATA3 gave non-specific staining compared to the other antibody, but at least that antibody is working for you. I have issued a credit for the original purchase, with the assumption that you will need an Abcam anti...

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OK, I have left the order in place. I expect it will be delivered early next week.

We encourage you to send a review of your mouse and/or human results, positive or negative, via our Abreview program. Reviews earn credits towards future purc...

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We do not have any sample sizes of this or any of our other antibodies. If you have human xenografts that you were planning on testing anyway, those would serve as positive controls, and would allow me to issue a refund for the original order if ab1135...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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