概述

  • 产品名称Anti-GATA3抗体
    参阅全部 GATA3 一抗
  • 描述
    兔多克隆抗体to GATA3
  • 特异性At least two isoforms of GATA3 are known to exist; ab106625 will recognize both isoforms.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human GATA3 (C terminal).
    Database link: P23771

  • 阳性对照
    • Human brain tissue lysate, Rat brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab106625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2.5 - 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.

靶标

  • 功能Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • 组织特异性T-cells and endothelial cells.
  • 疾病相关Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • 序列相似性Contains 2 GATA-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 形式There are 2 isoforms produced by alternative splicing.
  • 别名
    • GATA 3 antibody
    • GATA binding factor 3 antibody
    • GATA binding protein 3 antibody
    • GATA-binding factor 3 antibody
    • Gata3 antibody
    • GATA3_HUMAN antibody
    • HDR antibody
    • HDRS antibody
    • MGC2346 antibody
    • MGC5199 antibody
    • MGC5445 antibody
    • Trans acting T cell specific transcription factor GATA 3 antibody
    • Trans-acting T-cell-specific transcription factor GATA-3 antibody
    see all

Anti-GATA3 antibody 图像

  • Lane 1 : Anti-GATA3 antibody (ab106625) at 1 µg/ml
    Lane 2 : Anti-GATA3 antibody (ab106625) at 2 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 48 kDa
  • ab106625, at 2.5 ug/ml, staining GATA3 in paraffin embedded Rat brain tissue by Immunohistochemistry.
  • Immunohistochemical analysis of rat brain tissue labelling GATA3 with ab106625 at 20 ug/ml.

Anti-GATA3 antibody (ab106625)参考文献

This product has been referenced in:
  • Kim TH  et al. Identification of Creb3l4 as an essential negative regulator of adipogenesis. Cell Death Dis 5:e1527 (2014). WB ; Mouse . Read more (PubMed: 25412305) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 80 µg
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Sample Human Cell lysate - whole cell (Jurkat cell lysate)
Specification Jurkat cell lysate
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 24 2015

Thank you for contacting us. I have checked our catalog and found 4 anti-GATA3 antibodies that have been tested in mouse (ab32858, ab106625, ab82828 and ab110093), however none of these antibodies has been tested so far in frozen tissue section...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"