Anti-GATA2抗体- ChIP Grade (ab22849)

概述

  • 产品名称Anti-GATA2抗体- ChIP Grade
    参阅全部 GATA2 一抗
  • 描述
    兔多克隆抗体to GATA2 - ChIP Grade
  • 经测试应用适用于: EMSA, ChIP, IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: PYYANPAHAR ARVSYSPAHA RLTGGQMCRP HLLHSPGLPW LDGGKAALSA , corresponding to N terminal amino acids 58-107 of Human GATA2

  • 阳性对照
    • Human liver tissue (IHC-P), K682 cell lysate (western blot).

性能

应用

Our Abpromise guarantee covers the use of ab22849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
EMSA Use at an assay dependent concentration. PubMed: 20599521
ChIP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr 1/1000.

靶标

Anti-GATA2 antibody - ChIP Grade 图像

  • Anti-GATA2 antibody - ChIP Grade (ab22849) at 4 µg/ml + K682 (liver) cell lysate

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human liver tissue labelling Cata2 with ab22849 at a concentration of 4-8µg/ml. Arrows indicate positive labelled hepatocytes. Magnification: 400x.

  • ab22849 staining GATA2 in Zebrafish nervous system by Immunohistochemistry (Frozen sections).

    Tissue was fixed with paraformaldehyde and permeabilized with 0.3% Triton X-100. Samples were incubated with primary antibody (1/1000 in PBS + 0.3% Triton X-100) for 16 hours at 4°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

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Anti-GATA2 antibody - ChIP Grade (ab22849)参考文献

This product has been referenced in:
  • Guaraldo M  et al. Characterization of human mitochondrial ferritin promoter: identification of transcription factors and evidences of epigenetic control. Sci Rep 6:33432 (2016). ChIP ; Human . Read more (PubMed: 27625068) »
  • Majumder A  et al. Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1. J Biol Chem 290:13053-63 (2015). Read more (PubMed: 25847244) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Zebrafish Tissue sections (central nervous system)
Specification central nervous system
Fixative Paraformaldehyde
Permeabilization Yes - PBS + 0.3 % Triton X-100
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Abcam user community

Verified customer

提交于 Sep 14 2012

Thank you very much for your interest in ab22849.
To our knowledge, ab22849 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab22849 now, test it in mouse and submit feedback to us in the form of an ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (Lung cancer cells)
Specification Lung cancer cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Semiquantitative PCR
Positive control 1% input total chromatin DNA.
Negative control ChIP with beads and IgG only.
Username

Abcam user community

Verified customer

提交于 Mar 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (treated bone marrow cells)
Loading amount 30 µg
Specification treated bone marrow cells
Treatment virus
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 01 2008

Thank you for your enquiry. We are not aware of a publication using this antibody in staining fixed tissues. The image on the datasheet is from testing the antibody, and is not published. We guarantee that our products will perform as stated on ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"