概述

  • 产品名称Anti-GATA1抗体
    参阅全部 GATA1 一抗
  • 描述
    兔多克隆抗体to GATA1
  • 特异性ab28839 recognises GATA-1.
  • 经测试应用适用于: WB, ELISA, IHC-P, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 142.

  • 阳性对照
    • IHC: breast carcinoma WB: K562 cell line

性能

应用

Our Abpromise guarantee covers the use of ab28839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
  • 组织特异性Erythrocytes.
  • 疾病相关Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
    Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
    Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.
  • 序列相似性Contains 2 GATA-type zinc fingers.
  • 结构域The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.
  • 翻译后修饰Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
    Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anemia, X-linked, without thrombocytopenia, included antibody
    • ERYF 1 antibody
    • Eryf1 antibody
    • Erythroid transcription factor antibody
    • Erythrold transcription factor 1 antibody
    • GATA 1 antibody
    • GATA binding factor 1 antibody
    • GATA binding protein 1 (globin transcription factor 1) antibody
    • GATA binding protein 1 antibody
    • GATA-1 antibody
    • GATA-binding factor 1 antibody
    • GATA1 antibody
    • GATA1_HUMAN antibody
    • GF 1 antibody
    • GF-1 antibody
    • GF1 antibody
    • Globin transcription factor 1 antibody
    • NF E1 antibody
    • NF E1 DNA binding protein antibody
    • NF-E1 DNA-binding protein antibody
    • NFE 1 antibody
    • NFE1 antibody
    • Nuclear factor erythroid 1 antibody
    • Transcription factor GATA1 antibody
    • XLANP antibody
    • XLTDA antibody
    • XLTT antibody
    see all

Anti-GATA1 antibody 图像

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using GATA-1(Ab-1) Antibody (ab28839); Right: The same antibody preincubated with synthesized peptide.
  • All lanes : Anti-GATA1 antibody (ab28839) (1/500-1/1000 dilution)

    Lane 1 : extracts from K562 cells (5-30ug).
    Lane 2 : extracts from K562 cells (5-30ug).


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • ICC/IF image of ab28839 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28839, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GATA1 antibody (ab28839)参考文献

This product has been referenced in:
  • Fu W  et al. A multiply redundant genetic switch 'locks in' the transcriptional signature of regulatory T cells. Nat Immunol 13:972-80 (2012). Read more (PubMed: 22961053) »
  • Boidot R  et al. The transcription factor GATA-1 is overexpressed in breast carcinomas and contributes to survivin upregulation via a promoter polymorphism. Oncogene 29:2577-84 (2010). WB, IHC-P ; Human . Read more (PubMed: 20101202) »

See all 4 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (several cell lines)
Loading amount 20 µg
Specification several cell lines
Gel Running Conditions Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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Verified customer

提交于 Nov 26 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Bone marrow cell (linage negative))
Loading amount 10 µg
Specification Bone marrow cell (linage negative)
Treatment Treated 2 days before harvesting with 5FU (150 mg/Kg of body weight)
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 20 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"