Anti-Gastrin Releasing肽抗体(ab22623)

概述

  • 产品名称Anti-Gastrin Releasing肽抗体
    参阅全部 Gastrin Releasing Peptide 一抗
  • 描述
    兔多克隆抗体to Gastrin Releasing肽
  • 特异性ab22623 recognises Gastrin Releasing Peptide
  • 经测试应用适用于: ICC/IF, IHC-FoFr, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
  • 免疫原

    Synthetic GRP conjugated to BSA by a Glutaraldehyde linker.

  • 阳性对照
    • Pulmonary neuroendocrine cells

性能

应用

Our Abpromise guarantee covers the use of ab22623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/20.
IHC-FoFr 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 22411014
IHC-Fr 1/5000.

靶标

Anti-Gastrin Releasing Peptide antibody 图像

  • ab22623 at a 1/1000 dilution staining mouse brain (SCN region) by Immunohistochemistry (Formalin fixed sections). The antibody was incubated with the tissue for 16 hours and then detected using an Alexa-Fluor® 488 conjuaged donkey anti-rabbit polyclonal antibody. The mouse SCN region was isolated by stain with Guinea Pig anti-Vasoactive Intestinal Peptide (VIP) antibody.

    This image is courtesy of an Abreview by Gui-lan Yao submitted on 30 November 2005.

    See Abreview

Anti-Gastrin Releasing Peptide antibody (ab22623)参考文献

This product has been referenced in:
  • Tattersall M  et al. Expression of gastrin-releasing peptide is increased by prolonged stretch of human myometrium, and antagonists of its receptor inhibit contractility. J Physiol 590:2081-93 (2012). IHC-P ; Human . Read more (PubMed: 22411014) »
  • Rovsing L  et al. A neuroanatomical and physiological study of the non-image forming visual system of the cone-rod homeobox gene (Crx) knock out mouse. Brain Res 1343:54-65 (2010). IHC-Fr ; Mouse . Read more (PubMed: 20438719) »

See all 4 Publications for this product

Product Wall

Hello!

In order for you to successfully use a conjugation kit such as ab102925 with an antibody that is from whole serum (such as the GRP antibody such as ab22623) you will first need to purify the antibody to remove additional proteins that t...

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I am sorry but for neat serum in particular, there will not be any concentration indicated at all. Even trying to measure it via NanoDrop is not useful simply
because neat serum is typically too “dirty” (extremely protein rich and too ...

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Thank you for your reply. I am sorry that I could not be more helpful in your search for antibodies towards the proteins Retinoic acid and 9-cis Retinoic acid. If I do find any I will send that information along to you. If there is ever anything that I...

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Thank you for contacting Abcam. I have looked at all of the Gastrin Releasing Peptide antibodies in our catalogue and they are all polyclonal raised against the full length (148aa) protein. Therefore I cannot guarantee that any if they will be able to ...

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Thank you for getting back to me. The following website has a great dot blotting protocol: http://www.westernblotting.org/dot%20blot%20protocol.htm I hope this information helps, please do not hesitate to contact us if you need any more advic...

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Thank you for your enquiry. Gastrin Releasing Peptide antibody (ab22623) has yet to be tested by western blotting. However, according to the SwissProt entry (P63153) the predicted molecular weight of porcine Gastrin Releasing Peptide is 2.8KDa. I w...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (mouse brain SCN region)
Specification mouse brain SCN region
Fixative Paraformaldehyde
Antigen retrieval step None
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Username

Dr. Gui-lan Yao

Verified customer

提交于 Nov 30 2005

According to UnitProt the MW of GRP is 2806 Da [This is the MW of the unprocessed precursor], Please let us know if you need any further information,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"