概述

  • 产品名称
    Anti-Gastrin抗体
    参阅全部 Gastrin 一抗
  • 描述
    兔多克隆抗体to Gastrin
  • 特异性
    ab53085 detects endogenous levels of total Gastrin protein. The immunogen for this antibody has high alignment to CKK. It is therefore predicted to detect CKK as well as Gastrin, though this has not been tested experimentally.
  • 经测试应用
    适用于: IHC-Fr, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from Human Gastrin

  • 阳性对照
    • NIH/3T3 cell extract

性能

应用

Our Abpromise guarantee covers the use of ab53085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 21233276
WB 1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
ELISA 1/10000.

靶标

  • 功能
    Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
  • 序列相似性
    Belongs to the gastrin/cholecystokinin family.
  • 翻译后修饰
    Two different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71.
    Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Component II antibody
    • G14 antibody
    • G17 antibody
    • G34 antibody
    • G52 antibody
    • G6 antibody
    • GAS antibody
    • Gast antibody
    • GAST_HUMAN antibody
    • Gastrin antibody
    • Gastrin component I antibody
    • Gastrin component II antibody
    • Gastrin component III antibody
    • Gastrin precursor antibody
    • Gastrin-14 antibody
    • Gastrin-17 antibody
    • Gastrin-34 antibody
    • Gastrin-52 antibody
    • Gastrin-6 antibody
    • Gastrin-71 antibody
    see all

图片

  • All lanes : Anti-Gastrin antibody (ab53085) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with peptide


    Predicted band size : 11 kDa
    Observed band size : 11 kDa

文献

This product has been referenced in:
  • Lavoie EG  et al. Ectonucleotidases in the digestive system: focus on NTPDase3 localization. Am J Physiol Gastrointest Liver Physiol 300:G608-20 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21233276) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your enquiry.

I am sorry to confirm that the crossreactivity of this ab53085 Gastrin antibody with CKK has not been specifically tested in the laboratory.

However, alignment of the immunogen sequence of the antibody ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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