RabMAb

Anti-GAS2抗体[EPR1742(2)] (ab109762)

概述

  • 产品名称Anti-GAS2抗体[EPR1742(2)]
    参阅全部 GAS2 一抗
  • 描述
    兔单克隆抗体[EPR1742(2)] to GAS2
  • 经测试应用适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the N-terminus in Human GAS2.

  • 阳性对照
    • HepG2, fetal liver, fetal kidney, and Jurkat lysates, Human stomach tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
    • 组织特异性Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
    • 序列相似性Belongs to the GAS2 family.
      Contains 1 CH (calponin-homology) domain.
      Contains 1 GAR domain.
    • 发展阶段Specifically expressed at growth arrest.
    • 翻译后修饰Cleaved, during apoptosis, on a specific aspartic residue by caspases.
      Phosphorylated on serine residues during the G0-G1 transition phase.
    • 细胞定位Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GAS 2 antibody
      • GAS-2 antibody
      • Gas2 antibody
      • GAS2_HUMAN antibody
      • Growth arrest specific 2 antibody
      • Growth arrest specific protein 2 antibody
      • Growth arrest-specific protein 2 antibody
      • MGC32610 antibody
      see all

    Anti-GAS2 antibody [EPR1742(2)] 图像

    • All lanes : Anti-GAS2 antibody [EPR1742(2)] (ab109762) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 35 kDa
      Observed band size : 35 kDa
    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue using ab109762 at a dilution of 1/50.

    Anti-GAS2 antibody [EPR1742(2)] (ab109762)参考文献

    ab109762 has not yet been referenced specifically in any publications.

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