The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
1/10 - 1/100.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明Is unsuitable for Flow Cyt or ICC.
功能May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
组织特异性Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
序列相似性Belongs to the GAS2 family. Contains 1 CH (calponin-homology) domain. Contains 1 GAR domain.
发展阶段Specifically expressed at growth arrest.
翻译后修饰Cleaved, during apoptosis, on a specific aspartic residue by caspases. Phosphorylated on serine residues during the G0-G1 transition phase.
细胞定位Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.