概述

  • 产品名称
    Anti-GAS2抗体[EPR1742(2)]
    参阅全部 GAS2 一抗
  • 描述
    兔单克隆抗体[EPR1742(2)] to GAS2
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the N-terminus in Human GAS2.

  • 阳性对照
    • HepG2, fetal liver, fetal kidney, and Jurkat lysates, Human stomach tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab109762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能
      May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
    • 组织特异性
      Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
    • 序列相似性
      Belongs to the GAS2 family.
      Contains 1 CH (calponin-homology) domain.
      Contains 1 GAR domain.
    • 发展阶段
      Specifically expressed at growth arrest.
    • 翻译后修饰
      Cleaved, during apoptosis, on a specific aspartic residue by caspases.
      Phosphorylated on serine residues during the G0-G1 transition phase.
    • 细胞定位
      Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GAS 2 antibody
      • GAS-2 antibody
      • Gas2 antibody
      • GAS2_HUMAN antibody
      • Growth arrest specific 2 antibody
      • Growth arrest specific protein 2 antibody
      • Growth arrest-specific protein 2 antibody
      • MGC32610 antibody
      see all

    Anti-GAS2 antibody [EPR1742(2)] 图像

    • All lanes : Anti-GAS2 antibody [EPR1742(2)] (ab109762) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 35 kDa
      Observed band size : 35 kDa
    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue using ab109762 at a dilution of 1/50.

    Anti-GAS2 antibody [EPR1742(2)] (ab109762)参考文献

    This product has been referenced in:
    • Sun L  et al. GAS2-Calpain2 axis contributes to the growth of leukemic cells. Acta Biochim Biophys Sin (Shanghai) 47:795-804 (2015). Read more (PubMed: 26358320) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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