概述

  • 产品名称Anti-GAS2抗体
    参阅全部 GAS2 一抗
  • 描述
    兔多克隆抗体to GAS2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Cow, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GAS2.

  • 阳性对照
    • This antibody gave a positive signal in Human and Mouse Kidney tissue lysate as well as both HEK293 and HepG2 whole cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

靶标

  • 功能May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
  • 组织特异性Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
  • 序列相似性Belongs to the GAS2 family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 GAR domain.
  • 发展阶段Specifically expressed at growth arrest.
  • 翻译后修饰Cleaved, during apoptosis, on a specific aspartic residue by caspases.
    Phosphorylated on serine residues during the G0-G1 transition phase.
  • 细胞定位Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GAS 2 antibody
    • GAS-2 antibody
    • Gas2 antibody
    • GAS2_HUMAN antibody
    • Growth arrest specific 2 antibody
    • Growth arrest specific protein 2 antibody
    • Growth arrest-specific protein 2 antibody
    • MGC32610 antibody
    see all

Anti-GAS2 antibody 图像

  • All lanes : Anti-GAS2 antibody (ab106505) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Kidney (Mouse) Tissue Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 73 kDa,89 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-GAS2 antibody (ab106505)参考文献

ab106505 has not yet been referenced specifically in any publications.

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