Anti-GAS 6抗体(ab85388)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to GAS 6
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human GAS 6.

    (Peptide available as ab97363.)

  • 阳性对照
    • This antibody gave a positive signal in Human Heart Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab85388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa).

靶标

  • 功能
    Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.
  • 组织特异性
    Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen.
  • 序列相似性
    Contains 4 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 laminin G-like domains.
  • 翻译后修饰
    Isoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL.
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AXL receptor tyrosine kinase ligand antibody
    • AXL stimulatory factor antibody
    • AXLLG antibody
    • AXS F antibody
    • AXSF antibody
    • DKFZp666G247 antibody
    • FLJ34709 antibody
    • Gas 6 antibody
    • GAS-6 antibody
    • Gas6 antibody
    • GAS6_HUMAN antibody
    • Growth arrest specific 6 antibody
    • Growth arrest-specific protein 6 antibody
    • Growth-arrest-specific protein 6 antibody
    • Growth-arrest-specific protein 6 precursor antibody
    see all

图片

  • Anti-GAS 6 antibody (ab85388) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 79 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    We hypothesize that the 75 kDa band represents the mature form of GAS 6, due to the removal of a 30 amino acid signal pepide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

实验方案

文献

ab85388 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 22 2014

Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (primary human macrophages)
Specification
primary human macrophages
Treatment
MDP
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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