概述

  • 产品名称
  • 描述
    兔多克隆抗体to GARS
  • 特异性
    This antibody reacts specifically with 79kDa GARS protein.
  • 经测试应用
    适用于: ICC/IF, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human)

  • 阳性对照
    • HEK293 cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: Whole serum
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab42905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/3000 - 1/10000.
IP 1/3000 - 1/10000.
WB 1/5000. Predicted molecular weight: 83 kDa.

靶标

  • 相关性
    GARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease.
  • 细胞定位
    Cytoplasm. Mitochondrion.
  • 数据库链接
  • 别名
    • AP 4 A synthetase antibody
    • Charcot Marie Tooth neuropathy 2D antibody
    • Charcot Marie Tooth neuropathy neuronal type D antibody
    • CMT2D antibody
    • Diadenosine tetraphosphate synthetase antibody
    • DSMAV antibody
    • EC 6.1.1.14 antibody
    • Glycine tRNA ligase antibody
    • Glycyl tRNA synthetase antibody
    • GlyRS antibody
    • HMN5 antibody
    • SMAD1 antibody
    see all

Anti-GARS antibody 图像

  • Anti-GARS antibody (ab42905) at 1/5000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 83 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab42905 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42905, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab42905 staining GARS in murine sciatic nerve tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% goat serum in PBS with 0.5% Triton X-100 for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in diluent) overnight at 4°C. An AlexaFluor®555-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

Anti-GARS antibody (ab42905)参考文献

This product has been referenced in:
  • Motley WW  et al. Charcot-Marie-Tooth-linked mutant GARS is toxic to peripheral neurons independent of wild-type GARS levels. PLoS Genet 7:e1002399 (2011). WB, IHC-FoFr ; Mouse . Read more (PubMed: 22144914) »
  • Vellaichamy A  et al. Proteomic interrogation of androgen action in prostate cancer cells reveals roles of aminoacyl tRNA synthetases. PLoS One 4:e7075 (2009). WB ; Human . Read more (PubMed: 19763266) »

See all 2 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Aug 21 2014

Application
Western blot
Sample
Rat Cell lysate - whole cell (C6 Rat glioma cells)
Loading amount
40 µg
Specification
C6 Rat glioma cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 12 2012


Thank you for your inquiry. We are happy to provide the discount code to the customer for testing ab42905 in rat. As you know, this code will give you: 1 free primary antibody before the expiration date. To redeem this offer, the customer must sub...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (neuron)
Total protein in input
15 µg
Specification
neuron
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

提交于 May 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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