概述

  • 产品名称Anti-GARS抗体
    参阅全部 GARS 一抗
  • 描述
    兔多克隆抗体to GARS
  • 特异性This antibody reacts specifically with 79kDa GARS protein.
  • 经测试应用适用于: ICC/IF, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human)

  • 阳性对照
    • HEK293 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab42905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/3000 - 1/10000.
IP 1/3000 - 1/10000.
WB 1/5000. Predicted molecular weight: 83 kDa.

靶标

  • 相关性GARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease.
  • 细胞定位Cytoplasm. Mitochondrion.
  • 数据库链接
  • 别名
    • AP 4 A synthetase antibody
    • Charcot Marie Tooth neuropathy 2D antibody
    • Charcot Marie Tooth neuropathy neuronal type D antibody
    • CMT2D antibody
    • Diadenosine tetraphosphate synthetase antibody
    • DSMAV antibody
    • EC 6.1.1.14 antibody
    • Glycine tRNA ligase antibody
    • Glycyl tRNA synthetase antibody
    • GlyRS antibody
    • HMN5 antibody
    • SMAD1 antibody
    see all

Anti-GARS antibody 图像

  • Anti-GARS antibody (ab42905) at 1/5000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 83 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab42905 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42905, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab42905 staining GARS in murine sciatic nerve tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% goat serum in PBS with 0.5% Triton X-100 for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in diluent) overnight at 4°C. An AlexaFluor®555-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

Anti-GARS antibody (ab42905)参考文献

This product has been referenced in:
  • Motley WW  et al. Charcot-Marie-Tooth-linked mutant GARS is toxic to peripheral neurons independent of wild-type GARS levels. PLoS Genet 7:e1002399 (2011). WB, IHC-FoFr ; Mouse . Read more (PubMed: 22144914) »
  • Vellaichamy A  et al. Proteomic interrogation of androgen action in prostate cancer cells reveals roles of aminoacyl tRNA synthetases. PLoS One 4:e7075 (2009). WB ; Human . Read more (PubMed: 19763266) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton-X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Aug 21 2014

Application Western blot
Sample Rat Cell lysate - whole cell (C6 Rat glioma cells)
Loading amount 40 µg
Specification C6 Rat glioma cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 12 2012


Thank you for your inquiry. We are happy to provide the discount code to the customer for testing ab42905 in rat. As you know, this code will give you: 1 free primary antibody before the expiration date. To redeem this offer, the customer must sub...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (neuron)
Total protein in input 15 µg
Specification neuron
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

提交于 May 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"