amino acids 737-828 (LVQPSASDFQ FCWNQAPYAI VCAFPYLLAF TTDSMEIRLV VNGNLVHTAV VPQLQLVASR SDIYFTATAA VNEVSSGGSS KGASARNSPQ TP) of Human GARNL3 (Q5VVW2).
Human nasopharynx tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/20 - 1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Belongs to the GARNL3 family.
Contains 1 CNH domain. Contains 1 Rap-GAP domain.
Information by UniProt
GTPase-activating Rap/Ran-GAP domain-like protein 3 antibody
Immunocytochemistry/ Immunofluorescence - Anti-GARNL3 antibody (ab122015)
Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles. Recommended concentration of ab122015 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL3 antibody (ab122015)
ab122015 staining GARNL3 in paraffin-embedded Human nasopharynx tissue by Immunohistochemistry.
Western blot - Anti-GARNL3 antibody (ab122015)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
has not yet been referenced specifically in any publications.
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