Anti-GAPDH抗体[GA1R] (ab125247)

概述

  • 产品名称
    Anti-GAPDH抗体[GA1R]
    参阅全部 GAPDH 一抗
  • 描述
    小鼠单克隆抗体[GA1R] to GAPDH
  • 特异性
    ab125247 recognizes native and denatured forms of GAPDH.
  • 经测试应用
    适用于: WB, Dot blot, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Other
    预测可用于: a wide range of other species
  • 免疫原

    Recombinant GAPDH.

  • 阳性对照
    • Human, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
  • 常规说明

    Store at 4°C (add 0.05% NaN3) for several days to weeks.

性能

应用

Our Abpromise guarantee covers the use of ab125247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

靶标

Anti-GAPDH antibody [GA1R] 图像

  • All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilution

    Lane 1 : Human tissue lysate
    Lane 2 : Mouse tissue lysate
    Lane 3 : Rat tissue lysate
    Lane 4 : Rabbit tissue lysate
    Lane 5 : Chicken tissue lysate
    Lane 6 : Hamster tissue lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique

    Predicted band size : 36 kDa
  • All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilution

    Lane 1 : BL21 bacterial lysate
    Lane 2 : Sf9 insect lysate
    Lane 3 : Saccharomyces cerevisiae lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique

    Predicted band size : 36 kDa

实验方案

Anti-GAPDH antibody [GA1R] (ab125247)参考文献

This product has been referenced in:
  • Ye Y  et al. microRNA-605 promotes cell proliferation, migration and invasion in non-small cell lung cancer by directly targeting LATS2. Exp Ther Med 14:867-873 (2017). Read more (PubMed: 28673012) »
  • Zeng Q  et al. Overexpression of miR-155 promotes the proliferation and invasion of oral squamous carcinoma cells by regulating BCL6/cyclin D2. Int J Mol Med 37:1274-80 (2016). WB . Read more (PubMed: 26986233) »

See all 16 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1, A549, HEK293, CEMx-174)
Gel Running Conditions
Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount
5 µg
Treatment
Cells were mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification
THP-1, A549, HEK293, CEMx-174
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

提交于 Oct 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
3.75e+006 cells
Gel Running Conditions
Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
Sample
Schizosaccharomyces pombe Cell lysate - whole cell (whole cell extract)
Specification
whole cell extract
Blocking step
0.5% BSA + 5% non fat milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jul 17 2013

Thank you for that feedback. I am pleased to hear that the replacement antibody has proved successful for you.

I do not completely understand why at a dilution of 1/500 you would only be able to perform two western blots. There should be enoug...

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I am very sorry for the delay in getting back to you in regards to the Western blotting protocol used with the Anti-GAPDH antibody [GA1R] ab125247.

I can now confirm that the following conditions were used to obtain the image presented on the ...

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Thank you very much for contacting Abcam.

To our knowledge, ab125247 has not been tested in Fly/Drosophila tissue. Therefore, I can offer you our AbTrial program where you can now use our products in an untested application or species without ...

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Thank you for your reply.

I would be concerned that you are getting a colour change in your Bradford reagent without any sample present. This would suggest that there is a contamination in your buffers. I would make new sterile ones to make s...

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Thank you for your reply.

I think it is very important to check how much protein is being loaded to keep it within a manageable range, loading too much can lead to aggregation of proteins and high background whilst loading too little can obvi...

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Thank you for confirming that information.

It would be helpful to have the order number if possible. I have found one order of the 17th of July delivered to the University of Copenhagen, Faculty of Health Sciences. Is this your order?
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Thank you for sharing this information.

I am currently consulting with the source of this antibody to try to understand why the results have not been as expected with this antibody.

In order to investigate the situation further, cou...

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Thank you for contacting us. I am very glad to hear that you have taken advantage of our 100% Abreview program.

I have found the review which you have submitted and wish to thank you for this. In order to take advantage of the review code,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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