Anti-GAPDH抗体- Carboxyterminal end (ab62489)

概述

  • 产品名称Anti-GAPDH抗体- Carboxyterminal end
    参阅全部 GAPDH 一抗
  • 描述
    兔多克隆抗体to GAPDH - Carboxyterminal end
  • 特异性This antibody reacts with GAPDH
  • 经测试应用适用于: ICC/IF, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 16 amino acid synthetic peptide from near the carboxy-terminus of human GAPDH

  • 阳性对照
    • Hela cell lysates

应用

Our Abpromise guarantee covers the use of ab62489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).

靶标

  • 功能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 序列相似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻译后修饰S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 细胞定位Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Aging associated gene 9 protein antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL S 162eP antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    see all

Anti-GAPDH antibody - Carboxyterminal end 图像

  • Lane 1 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 0.5 µg/ml
    Lane 2 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 1 µg/ml
    Lane 3 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 2 µg/ml

    Lane 1 : Hela cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Hela cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 36 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • ab62489 at 10µg/ml staining GAPDH in human Hela cells by Imunocytochemistry. 

  • Immunofluorescence of GAPDH in HeLa cells using ab62489 at 20 ug/ml.

Anti-GAPDH antibody - Carboxyterminal end (ab62489)参考文献

ab62489 has not yet been referenced specifically in any publications.

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