概述

  • 产品名称Anti-GAPDH抗体
    参阅全部 GAPDH 一抗
  • 描述
    兔多克隆抗体to GAPDH
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rabbit, Human
  • 免疫原

    Synthetic peptide corresponding to a portion of the Human GAPDH.

  • 阳性对照
    • HeLa cell lysate, 293 cell lysate, Jurkat cell lysate, THP1 cell lysate

应用

Our Abpromise guarantee covers the use of ab89422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 36 kDa.

靶标

  • 功能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 序列相似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻译后修饰S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 细胞定位Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt
  • 数据库链接
  • 别名
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    • HEL-S-162eP antibody
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Anti-GAPDH antibody 图像

  • All lanes : Anti-GAPDH antibody (ab89422) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : THP1 cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 36 kDa

Anti-GAPDH antibody (ab89422)参考文献

ab89422 has not yet been referenced specifically in any publications.

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