概述

  • 产品名称
  • 描述
    兔多克隆抗体to GAPDH
  • 特异性
    We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Gerbil, Chinese hamster
  • 免疫原

    Synthetic peptide within Mouse GAPDH aa 300-335 (C terminal). The exact sequence is proprietary.
    Database link: P16858

  • 阳性对照
    • Whole cell lysate from mouse NIH3T3 cells.

应用

Our Abpromise guarantee covers the use of ab70700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
    • 通路
      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
    • 序列相似性
      Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    • 翻译后修饰
      S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
      ISGylated.
    • 细胞定位
      Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
      • aging associated gene 9 protein antibody
      • Aging-associated gene 9 protein antibody
      • BARS-38 antibody
      • cb609 antibody
      • EC 1.2.1.12 antibody
      • Epididymis secretory sperm binding protein Li 162eP antibody
      • G3P_HUMAN antibody
      • G3PD antibody
      • G3PDH antibody
      • GAPD antibody
      • GAPDH antibody
      • Glyceraldehyde 3 phosphate dehydrogenase antibody
      • Glyceraldehyde-3-phosphate dehydrogenase antibody
      • HEL-S-162eP antibody
      • KNC-NDS6 antibody
      • MGC102544 antibody
      • MGC102546 antibody
      • MGC103190 antibody
      • MGC103191 antibody
      • MGC105239 antibody
      • MGC127711 antibody
      • MGC88685 antibody
      • OCAS, p38 component antibody
      • OCT1 coactivator in S phase, 38-KD component antibody
      • peptidyl cysteine S nitrosylase GAPDH antibody
      • Peptidyl-cysteine S-nitrosylase GAPDH antibody
      • wu:fb33a10 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse squamous cell carcinoma tissue labelling GAPDH with ab70700 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-GAPDH antibody (ab70700) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from mouse NIH3T3 cells. at 50 µg
      Lane 2 : Whole cell lysate from mouse NIH3T3 cells. at 15 µg
      Lane 3 : Whole cell lysate from mouse NIH3T3 cells. at 5 µg


      Predicted band size : 36 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

    文献

    ab70700 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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