概述

  • 产品名称Anti-GAPDH抗体
    参阅全部 GAPDH 一抗
  • 描述
    兔多克隆抗体to GAPDH
  • 特异性Can be used for the detection of GAPDH.
  • 经测试应用适用于: WB, IHC-P, ELISA, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Baboon
  • 免疫原

    Synthetic peptide, corresponding to 16 N terminal amino acids of Human GAPDH

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab37168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.

Only tested on peptide ELISA.

ICC/IF Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr 1/100.

靶标

  • 功能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 序列相似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻译后修饰S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 细胞定位Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Aging associated gene 9 protein antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL S 162eP antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    see all

Anti-GAPDH antibody 图像

  • Lane 1 : Anti-GAPDH antibody (ab37168) at 0.125 µg/ml
    Lane 2 : Anti-GAPDH antibody (ab37168) at 0.25 µg/ml
    Lane 3 : Anti-GAPDH antibody (ab37168) at 0.5 µg/ml

    Lane 1 : HeLa cell lysate with GAPDH peptide
    Lane 2 : HeLa cell lysate with GAPDH peptide
    Lane 3 : HeLa cell lysate with GAPDH peptide

    Lysates/proteins at 15 µg per lane.

    Secondary
    anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • ICC/IF image of ab37168 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1mg/ml) overnight at +4øC. The secondary antibody (green) was anti-rabbit Alexa Fluor® 488 (ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

  • ICC/IF image of ab37168 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1µg/ml) overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of GAPDH in HeLa cells using ab37168 at 10 ug/ml.

  • ab37168 at 10µg/ml staining GAPDH in human liver tissue by IHC

Anti-GAPDH antibody (ab37168)参考文献

This product has been referenced in:
  • Chen B  et al. Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Mol Med Rep 13:2527-35 (2016). WB ; Mouse . Read more (PubMed: 26848028) »
  • Liu J  et al. Long noncoding RNA POU6F2-AS2 is associated with oesophageal squamous cell carcinoma. J Biochem 160:195-204 (2016). Read more (PubMed: 27033944) »

See all 24 Publications for this product

Product Wall

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I am sorry that you were having issues with ab9485 in western blot. As we discussed earlier today, I am sending you ab37168 as a free of charge replacement. Your new order number is ******.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (lung)
Specification lung
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Mr. Pete Geisen

Verified customer

提交于 Jan 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (retina)
Specification retina
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Mr. Pete Geisen

Verified customer

提交于 Jan 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"