Anti-GAPDH抗体- Loading Control (ab22555)
Key features and details
- Rabbit polyclonal to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GAPDH抗体- Loading Control
参阅全部 GAPDH 一抗 -
描述
兔多克隆抗体to GAPDH - Loading Control -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Dog, Human, African green monkey
预测可用于: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Turkey, Pig, Duck -
免疫原
Synthetic peptide:
DAPMFVMGVNHFKYA
, corresponding to amino acids 126-140 of Human GAPDH. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22555于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (4) |
1/1000 - 1/5000. Detects a band of approximately 36 kDa.
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 36 kDa. |
靶标
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功能
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
序列相似性
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
翻译后修饰
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
细胞定位
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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数据库链接
- Entrez Gene: 493876 Cat
- Entrez Gene: 374193 Chicken
- Entrez Gene: 281181 Cow
- Entrez Gene: 403755 Dog
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 396823 Pig
see all -
别名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
图片
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Lane 1 : Anti-GAPDH antibody - Loading Control (ab22555)
Lanes 2-4 : Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 25 µg per lane. -
All lanes : Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : COS-7 cell lysate
Lane 3 : MDCK cell lysate
Lane 4 : PC-3 cell lysate
Lane 5 : Mouse brain tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ HRP conjugate at 1/4000 dilution
Observed band size: 37 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (106)
ab22555 被引用在 106 文献中.
- Pinheiro-de-Sousa I et al. Uncovering emergent phenotypes in endothelial cells by clustering of surrogates of cardiovascular risk factors. Sci Rep 12:1372 (2022). PubMed: 35079076
- Zhang Z et al. Icariin regulates stem cell migration for endogenous repair of intervertebral disc degeneration by increasing the expression of chemotactic cytokines. BMC Complement Med Ther 22:63 (2022). PubMed: 35272637
- Sathiyanadan K et al. Targeting Endothelial Connexin37 Reduces Angiogenesis and Decreases Tumor Growth. Int J Mol Sci 23:N/A (2022). PubMed: 35328350
- Ma X et al. TRAF2, an Innate Immune Sensor, Reciprocally Regulates Mitophagy and Inflammation to Maintain Cardiac Myocyte Homeostasis. JACC Basic Transl Sci 7:223-243 (2022). PubMed: 35411325
- Xu F et al. SLC1A5 Prefers to Play as an Accomplice Rather Than an Opponent in Pancreatic Adenocarcinoma. Front Cell Dev Biol 10:800925 (2022). PubMed: 35419359