Anti-GAPDH抗体[3E8AD9] - Loading Control (ab110305)
Key features and details
- Mouse monoclonal [3E8AD9] to GAPDH - Loading Control
- Suitable for: ICC/IF, WB, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-GAPDH抗体[3E8AD9] - Loading Control
参阅全部 GAPDH 一抗 -
描述
小鼠单克隆抗体[3E8AD9] to GAPDH - Loading Control -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WB, Flow Cyt, IPmore details -
种属反应性
与反应: Human -
免疫原
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2 cells IP: HepG2 cells and Human liver mitichondria Flow Cyt: HL-60 cells ICC/IF: Human HDFn cells
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯化说明
ab110305 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
3E8AD9 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110305于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 0.5 µg/ml.
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WB |
Use a concentration of 0.125 µg/ml. Predicted molecular weight: 36 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IP |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
Use a concentration of 0.5 µg/ml. |
WB
Use a concentration of 0.125 µg/ml. Predicted molecular weight: 36 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. |
靶标
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功能
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
序列相似性
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
翻译后修饰
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
细胞定位
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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数据库链接
- Entrez Gene: 2597 Human
- Omim: 138400 Human
- SwissProt: P04406 Human
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
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别名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
图片
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Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305) at 0.125 µg/ml + HepG2 cell lysate
Predicted band size: 36 kDa -
Immunocytochemistry analysis using ab110305 at 0.5 µg/ml to stain GAPDH in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized). Target locates primarily in the cytosol.
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Detection of ab110305 by Western Blot of Immunprecipitate.
Staining of GAPDH in HepG2 cells (lane 1) and Humman Liver Mitochondira (lane 2), immunoprecipitated using ab110305. -
Flow cytometric analysis of GAPDH in HL60 cells (blue), using ab110305 at 1 µg/ml. Isotype control antibody (red).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab110305 被引用在 10 文献中.
- Zhang C et al. Asiaticoside Suppresses Gastric Cancer Progression and Induces Endoplasmic Reticulum Stress through the miR-635/HMGA1 Axis. J Immunol Res 2022:1917585 (2022). PubMed: 35692504
- Cui Y et al. miRNA-193a-3p Regulates the AKT2 Pathway to Inhibit the Growth and Promote the Apoptosis of Glioma Cells by Targeting ALKBH5. Front Oncol 11:600451 (2021). PubMed: 33968717
- Jin S et al. 5-Azacitidine Induces NOXA to Prime AML Cells for Venetoclax-Mediated Apoptosis. Clin Cancer Res 26:3371-3383 (2020). PubMed: 32054729
- Men L et al. microRNA-577 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting homeobox A1. Mol Med Rep 19:1875-1882 (2019). PubMed: 30628697
- Zhao Z et al. MicroRNA-655 inhibits cell proliferation and invasion in epithelial ovarian cancer by directly targeting vascular endothelial growth factor. Mol Med Rep 18:1878-1884 (2018). PubMed: 29845206
- Li M et al. DNA damage-induced cell death relies on SLFN11-dependent cleavage of distinct type II tRNAs. Nat Struct Mol Biol 25:1047-1058 (2018). PubMed: 30374083
- Sun LL et al. Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces ß-cell proliferation in diabetic mice. Int J Mol Med 39:936-948 (2017). WB ; Human . PubMed: 28290605
- Parplys AC et al. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Res 43:9817-34 (2015). WB ; Human . PubMed: 26323318
- Lin X et al. Silencing of Livin inhibits tumorigenesis and metastasis via VEGF and MMPs pathway in lung cancer. Int J Oncol 47:657-67 (2015). PubMed: 26094984
- Whelan RS et al. Bax regulates primary necrosis through mitochondrial dynamics. Proc Natl Acad Sci U S A 109:6566-71 (2012). PubMed: 22493254