Anti-GAP43抗体- Neuronal Marker (ab16053)

概述

  • 产品名称Anti-GAP43抗体- Neuronal Marker
    参阅全部 GAP43 一抗
  • 描述
    兔多克隆抗体to GAP43 - Neuronal Marker
  • 特异性This antibody does not perform well in IHC-P (abreview 21090, 21089)
  • 经测试应用适用于: ICC/IF, IHC (PFA fixed), WB, IHC-Frmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Rat GAP43.

    (Peptide available as ab16379.)

  • 阳性对照
    • This antibody gave a positive signal in Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation as well as Mouse spinal cord tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab16053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
IHC (PFA fixed) 1/300 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43-53 kDa (predicted molecular weight: 43-53 kDa).Can be blocked with Rat GAP43 peptide (ab16379).
IHC-Fr 1/500.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
    • 序列相似性Belongs to the neuromodulin family.
      Contains 1 IQ domain.
    • 翻译后修饰Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
    • 细胞定位Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Axonal membrane protein GAP 43 antibody
      • Axonal membrane protein GAP-43 antibody
      • B 50 antibody
      • Calmodulin binding protein P 57 antibody
      • F1 antibody
      • GAP 43 antibody
      • GAP43 antibody
      • Growth Associated Protein 43 antibody
      • Growth-associated protein 43 antibody
      • Nerve Growth Related Peptide antibody
      • Nerve growth related peptide GAP43 antibody
      • NEUM_HUMAN antibody
      • Neural phosphoprotein B 50 antibody
      • Neural phosphoprotein B-50 antibody
      • Neuromodulin antibody
      • Neuron growth associated protein 43 antibody
      • PP46 antibody
      • Protein F1 antibody
      • QtrA-11580 antibody
      • QtrA-13071 antibody
      see all

    Anti-GAP43 antibody - Neuronal Marker 图像

    • Immunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379).

      Protocol details: Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20µm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.

    • All lanes : Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml

      Lane 1 : spinal cord chung rat 1
      Lane 2 : spinal cord chung rat 2
      Lane 3 : spinal cord chung rat 3

      Lysates/proteins at 20 µg per lane.

      Secondary
      Dako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilution

      Performed under reducing conditions.

      Predicted band size : 43-53 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 90 kDa (possible multimer).
    • Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 43-53 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa,50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 8 minutes
    • ab16053 staining GAP43 in murine dorsal root ganglia by Immunocytochemistry/ Immunofluorescence.
      Cells were fixed with methanol, permeabilized using 0.1% saponin/PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab16053 at a 1/500 dilution for 14 hours at 4°C. The secondary antibody used was a DyLight 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

      See Abreview

    Anti-GAP43 antibody - Neuronal Marker (ab16053)参考文献

    This product has been referenced in:
    • Nagai J  et al. Crmp4 deletion promotes recovery from spinal cord injury by neuroprotection and limited scar formation. Sci Rep 5:8269 (2015). WB, IHC-Fr ; Mouse . Read more (PubMed: 25652774) »
    • D'Agostino G  et al. Prolyl endopeptidase-deficient mice have reduced synaptic spine density in the CA1 region of the hippocampus, impaired LTP, and spatial learning and memory. Cereb Cortex 23:2007-14 (2013). Read more (PubMed: 22767632) »

    See all 10 Publications for this product

    Product Wall

    We would recommend using ab16053 in place of the discontinued item. Ab16053 has been tested in and is guaranteed in IF on mouse samples. This product has also been featured in 5 publications which we are aware of and has excellent Abreviews. More infor...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (mouse brain (hippocampus))
    Specification mouse brain (hippocampus)
    Fixative 4% PFA fixed
    Antigen retrieval step None
    Permeabilization Yes - 0,1% Saponin
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 19 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (mouse lung (bronchus))
    Specification mouse lung (bronchus)
    Fixative Methanol
    Permeabilization Yes - 0,1% Saponin
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (brain tissue)
    Loading amount 10 µg
    Specification brain tissue
    Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 09 2012

    Thank you for your reply.

    Your protocol looks good. The one recommendation I could make would be to increase the dilution of the primary and secondary (especially) to see if that improves the staining.
    You can try the primary at 1:250 a...

    Read More

    Thank you for calling us and for alerting us to the problem you are experiencing with anti-GAP43 antibody (ab16053). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    As we...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (dorsal root ganglia)
    Specification dorsal root ganglia
    Fixative Methanol
    Permeabilization Yes - 0,1% Saponin / PBS
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 12 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (in situ spinal cord)
    Specification in situ spinal cord
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Nov 02 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Head)
    Specification Head
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Nov 02 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - other (NG108-15 neuroblastoma x glioma (mouse/rat) hybrid)
    Loading amount 10 µg
    Specification NG108-15 neuroblastoma x glioma (mouse/rat) hybrid
    Treatment DMEM+HAT, with 5% FBS for 20 hrs
    Gel Running Conditions Reduced Denaturing (10% acrylamide)
    Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Alan Howe

    Verified customer

    提交于 Sep 23 2009

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