概述

  • 产品名称
  • 描述
    兔多克隆抗体to GAP43
  • 特异性
    This antibody recognises the protein designated Gap43.
  • 经测试应用
    适用于: IHC-FoFr, IHC-Fr, IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Cat, Human, Monkey
  • 免疫原

    Purified GAP43 from cat brain.

  • 常规说明
    This product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab11136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 25249946
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 43-57 kDa (predicted molecular weight: 28.6 kDa).
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
  • 序列相似性
    Belongs to the neuromodulin family.
    Contains 1 IQ domain.
  • 翻译后修饰
    Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
  • 细胞定位
    Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Axonal membrane protein GAP 43 antibody
    • Axonal membrane protein GAP-43 antibody
    • B 50 antibody
    • Calmodulin binding protein P 57 antibody
    • F1 antibody
    • GAP 43 antibody
    • GAP43 antibody
    • Growth Associated Protein 43 antibody
    • Growth-associated protein 43 antibody
    • Nerve Growth Related Peptide antibody
    • Nerve growth related peptide GAP43 antibody
    • NEUM_HUMAN antibody
    • Neural phosphoprotein B 50 antibody
    • Neural phosphoprotein B-50 antibody
    • Neuromodulin antibody
    • Neuron growth associated protein 43 antibody
    • PP46 antibody
    • Protein F1 antibody
    • QtrA-11580 antibody
    • QtrA-13071 antibody
    see all

文献

This product has been referenced in:
  • Dhillon RS  et al. Axonal plasticity underpins the functional recovery following surgical decompression in a rat model of cervical spondylotic myelopathy. Acta Neuropathol Commun 4:89 (2016). IHC-Fr ; Rat . Read more (PubMed: 27552807) »
  • Benitez SU  et al. Synaptic plasticity and sensory-motor improvement following fibrin sealant dorsal root reimplantation and mononuclear cell therapy. Front Neuroanat 8:96 (2014). IHC-FoFr ; Rat . Read more (PubMed: 25249946) »

See all 4 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (hippocampus)
Loading amount
10 µg
Specification
hippocampus
Gel Running Conditions
Non-reduced Denaturing (10%SDS gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

提交于 Apr 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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