Anti-Ganglioside GM1抗体(ab31119)

概述

  • 产品名称
    Anti-Ganglioside GM1抗体
    参阅全部 Ganglioside GM1 一抗
  • 描述
    兔多克隆抗体to Ganglioside GM1
  • 特异性
    Recognizes ganglioside GM1. Expresses some reactivity to asialo GM1 due to the natural background in rabbit. Does not crossreact with other carbohydrate epitopes.
  • 经测试应用
    适用于: ICC/IF, ELISA, Thin Layer Chromatographymore details
  • 免疫原

    Other Immunogen Type corresponding to Cow Ganglioside GM1. Immunogen=Purified monosialoganglioside GM1 and complete Freund’s adjuvant.

  • 常规说明
    Addition of glycerol as a cryopreservative is not recommended.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: Whole serum
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
ELISA 1/10000.
Thin Layer Chromatography 1/1000.

靶标

  • 相关性
    A member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function.
  • 细胞定位
    Plasma membrane
  • 别名
    • GM1 ganglioside antibody
    • Monosialoanglioside antibody

文献

ab31119 has not yet been referenced specifically in any publications.

客户评价及客户问答

The reason that species is not mentioned on the datasheet is because the target is species independent. The target is Ganglioside GM1, a molecule composed of a glycosphingolipid (ceramide and oligosaccharide) with sialic acids linked on the sugar chain...

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Thank you very much for your reply I am pleased to hear you are considering trying GM1 antibody ab31119 in ICC-IF.  Therefore, I am pleased to provide details of the testing discount program we can offer you: I can provide a discount off a future purch...

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Thank you for your enquiry. I can confirm these are different antibodies, which have been tested in different applications. Tested applications covered by our guarnatee are listed on teh online datasheets: ab23943  Anti-Ganglioside GM1 antibody...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Bronchial epithelial cells)
Specification
Bronchial epithelial cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 24°C
Username

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提交于 Mar 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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