Anti-gamma Tubulin抗体[TU-30] (ab27074)

概述

  • 产品名称Anti-gamma Tubulin抗体[TU-30]
    参阅全部 gamma Tubulin 一抗
  • 描述
    小鼠单克隆抗体[TU-30] to gamma Tubulin
  • 特异性This antibody recognises gamma tubulin expressed on stomach, kidney, liver, lung, muscle, ovary, heart, colon, thymus, brain.
  • 经测试应用适用于: Flow Cyt, IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human, Pig, Xenopus laevis, Chinese Hamster
    预测可用于: Plants
  • 免疫原

    Synthetic peptide:

    EYHAATRPDYISWGTQ

    conjugated to KLH, corresponding to amino acids 434-449 of gamma Tubulin.

  • 表位This antibody reacts with an epitope located in the C terminal region of gamma tubulin.
  • 阳性对照
    • P19 cell line, 3T3 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab27074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 2 µg/ml.

Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells.

WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.

靶标

  • 功能Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • 序列相似性Belongs to the tubulin family.
  • 翻译后修饰Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

Anti-gamma Tubulin antibody [TU-30] 图像

  • Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).

  • ab27074 staining gamma tubulin in human colon and thyroid tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in EDTA pH 9.0 without undergoing blocking step. The primary antibody was diluted 1/1000 and incubated with sample for 1 hour at room temperature. A HRP-conjugated goat polyclonal to mouse antibody was used undiluted, as secondary.

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  • ab27074 staining gamma tubulin in the Human HEK293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol (-20ºC 20 min) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 or 1/1000 in blocking solution) for 45 minutes. A Texas Red®-conjugated Goat anti-mouse polyclonal (1/500) was used as the secondary antibody.

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  • ab27074 staining gamma tubulin (red) in rat prostate carcinoma cells by ICC/IF. The cells were also transfected with beta-tubulin GFP which stained green.

  • Overlay histogram showing HeLa cells stained with ab27074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27074, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-gamma Tubulin antibody [TU-30] (ab27074)参考文献

This product has been referenced in:

See all 13 Publications for this product

Product Wall

Thank you for contacting us. This product has been tested for Immunohistochemistry. The reference is as follows:

Nicholas AK et al. WDR62 is associated with the spindle pole and is mutated in human microcephaly. Nat Genet 42:1010-4 (2010). IHC...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK 293T)
Specification HEK 293T
Fixative Paraformaldehyde
Permeabilization Yes - Methanol -20ºC 20 min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Nov 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon and Thyroid)
Specification Colon and Thyroid
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH9.0 EDTA
Username

Mr. 孝一 鎌田

Verified customer

提交于 Dec 11 2008

Thank you for your enquiry. I can tell you that this antibody has been applied in the following plant publication; Binarova P et al. Nuclear gamma-tubulin during acentriolar plant mitosis. Plant Cell 12:433-42 (2000). PubMed: 10715328 I have ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"