概述

  • 产品名称
    Anti-gamma Tubulin抗体
    参阅全部 gamma Tubulin 一抗
  • 描述
    兔多克隆抗体to gamma Tubulin
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Caenorhabditis elegans
  • 免疫原

    Synthetic peptide

    LDEYKAVVQKDYLTRGL

    with N-terminal added cysteine conjugated to KLH, corresponding to C terminal amino acids 428-444 of Caenorhabditis elegans gamma Tubulin

  • 阳性对照
    • Whole extract of C. elegans

性能

应用

Our Abpromise guarantee covers the use of ab50721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50.
WB Use a concentration of 0.75 - 1.5 µg/ml. Predicted molecular weight: 48 kDa.

靶标

  • 功能
    Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • 序列相似性
    Belongs to the tubulin family.
  • 翻译后修饰
    Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • 细胞定位
    Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 别名
    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

图片

  • All lanes : Anti-gamma Tubulin antibody (ab50721) at 1/2000 dilution

    Lane 1 : C. elegans whole extract
    Lane 2 : C. elegans whole extract with gamma Tubulin immunizing peptide at 20 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate
    Developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

文献

This product has been referenced in:
  • Kokona B  et al. Studying polyglutamine aggregation in Caenorhabditis elegans using an analytical ultracentrifuge equipped with fluorescence detection. Protein Sci 25:605-17 (2016). Read more (PubMed: 26647351) »
  • Wiese M  et al. Regulation of neuronal APL-1 expression by cholesterol starvation. PLoS One 7:e32038 (2012). WB ; Caenorhabditis elegans . Read more (PubMed: 22363792) »

See all 3 Publications for this product

客户评价及客户问答

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Caenorhabditis elegans Cell (embryo)
Specification
embryo
Fixative
Paraformaldehyde
Permeabilization
Yes - freeze-cracking and methanol (5 min at -20C)
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提交于 Feb 29 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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