This sequence is highly specific for C. elegans gamma tubulin, and is not found in gamma tubulin sequences of other species or other members of the tubulin family such as alpha, beta, delta and epsilon tubulins.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.75 - 1.5 µg/ml. Predicted molecular weight: 48 kDa.
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Belongs to the tubulin family.
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Kokona B et al. Studying polyglutamine aggregation in Caenorhabditis elegans using an analytical ultracentrifuge equipped with fluorescence detection. Protein Sci25:605-17 (2016).
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Wiese M et al. Regulation of neuronal APL-1 expression by cholesterol starvation. PLoS One7:e32038 (2012).
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