Anti-gamma Tubulin抗体(ab11321)

概述

  • 产品名称Anti-gamma Tubulin抗体
    参阅全部 gamma Tubulin 一抗
  • 描述
    兔多克隆抗体to gamma Tubulin
  • 特异性Does not react with other members of the tubulin family, such as alpha, beta, omega, and eta Tubulin.
  • 经测试应用适用于: IHC-P, IHC-Fr, ICC/IF, IHC (Methanol fixed), WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cat, Human, Pig, Xenopus laevis, Zebrafish
    预测可用于: Dog, Sea Urchin
  • 免疫原

    Synthetic peptide:

    AATRPDYISWGTQEQ-CG

    , with N-terminally added cysteine-glycine, conjugated to KLH, corresponding to amino acids 437-451 of Xenopus laevis gamma Tubulin.

  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

性能

应用

Our Abpromise guarantee covers the use of ab11321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/1000.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 19187565
IHC (Methanol fixed) 1/200. (Methanol/H2O2 fixed cells, agarose embedded): 1/200 (see PMID17329357 for further details).
WB 1/1000. Detects a band of approximately 48 kDa. (for whole cell extract of the human cell line HeLa and at a dilution of 1/5000 for Xenopus whole egg extract).

靶标

Anti-gamma Tubulin antibody 图像

  • All lanes : Anti-gamma Tubulin antibody (ab11321) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse hepatocytes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-rabbit (HRP) secondary antibody at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    Blocking buffer 5% milk.

    See Abreview

  • ICC/IF image of ab11321 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11321, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
  • ab11321 at a 1/200 dilution staining gamma Tubulin in Xenopus neuroepithelial cells by Immunohistochemistry. (A) High-magnification projection of a stack of optical sections showing elongating neural epithelial cells stained for ?-tubulin (white lines indicate cell outline). (a') a-tubulin staining for cells shown in A. (B) Schematic of the closing neural tube indicating location of section shown in A (red box). (C) ?-tubulin staining in a projection of optical sections reveals centrosome-like foci at the apical surfaces of mature neuroepithelial cells. (c') Acetylated-a-tubulin staining reveals cell outlines in the ventral region of the closed Xenopus neural tube. (D) Schematic of the neural tube indicating location of section shown in C (red box). (E) Optical section showing a-tubulin in an embryo unilaterally injected with Shroom3-MO (right side is injected, left side is control). (e') ?-tubulin staining for cells shown in E.

Anti-gamma Tubulin antibody (ab11321)参考文献

This product has been referenced in:
  • Brooks ER & Wallingford JB In vivo investigation of cilia structure and function using Xenopus. Methods Cell Biol 127:131-59 (2015). IHC ; Xenopus laevis . Read more (PubMed: 25837389) »
  • Itoh K  et al. GEF-H1 functions in apical constriction and cell intercalations and is essential for vertebrate neural tube closure. J Cell Sci 127:2542-53 (2014). Read more (PubMed: 24681784) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (liver, hepatocytes)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification liver, hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 15 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Embryonic Fibroblasts)
Specification Mouse Embryonic Fibroblasts
Fixative 1:1 methanol:acetone
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Dr. Gabriel Balmus

Verified customer

提交于 Feb 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (frozen section of brain choroid plexus)
Specification frozen section of brain choroid plexus
Fixative Paraformaldehyde
Permeabilization Yes - Triton X100
Username

Prof. Antonio J Jimenez

Verified customer

提交于 Jun 12 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Zebrafish Tissue sections (whole mount)
Specification whole mount
Fixative Acetone
Antigen retrieval step None
Permeabilization No
Blocking step goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 12 2007

Thank you for your enquiry. Details of the immunogens used for each of the antisera that you are interested in are as follows: I think that you may have mistyped the first antiserum as it is not directed against tubulin. CDCP1 antibody (ab1...

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Thank you for your enquiry regarding ab11321. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimburseme...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"