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Our Abpromise guarantee covers the use of ab11321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19187565|
|IHC (Methanol fixed)||1/200. (Methanol/H2O2 fixed cells, agarose embedded): 1/200 (see PMID17329357 for further details).|
|WB||1/1000. Detects a band of approximately 48 kDa. (for whole cell extract of the human cell line HeLa and at a dilution of 1/5000 for Xenopus whole egg extract).|
Blocking buffer 5% milk.
ab11321 at a 1/200 dilution staining gamma Tubulin in Xenopus neuroepithelial cells by Immunohistochemistry. (A) High-magnification projection of a stack of optical sections showing elongating neural epithelial cells stained for ?-tubulin (white lines indicate cell outline). (a') a-tubulin staining for cells shown in A. (B) Schematic of the closing neural tube indicating location of section shown in A (red box). (C) ?-tubulin staining in a projection of optical sections reveals centrosome-like foci at the apical surfaces of mature neuroepithelial cells. (c') Acetylated-a-tubulin staining reveals cell outlines in the ventral region of the closed Xenopus neural tube. (D) Schematic of the neural tube indicating location of section shown in C (red box). (E) Optical section showing a-tubulin in an embryo unilaterally injected with Shroom3-MO (right side is injected, left side is control). (e') ?-tubulin staining for cells shown in E.
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