gamma Synuclein overexpression 293T裂解液(全细胞) (ab94101)

概述

  • 产品名称gamma Synuclein overexpression 293T裂解液(全细胞)
  • 描述
    gamma Synuclein overexpression裂解液
  • 常规说明ab94101 is a 293T cell transfected lysate in which Human gamma Synuclein has been transiently over-expressed using a pCMV-gamma Synuclein plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. Tissue specificity: Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. Similarity: Belongs to the synuclein family. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.

应用

Our Abpromise guarantee covers the use of ab94101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

gamma Synuclein overexpression 293T lysate (whole cell) 图像

  • ab94101 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-gamma Synuclein antibody (ab89462) at 1/500 dilution

    Lane 1 : gamma Synuclein overexpression 293T lysate (whole cell) (ab94101)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

gamma Synuclein overexpression 293T lysate (whole cell) (ab94101)参考文献

ab94101 has not yet been referenced specifically in any publications.

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