gamma Synuclein/SNCG overexpression 293T裂解物(whole cell) (ab94101)
概述
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产品名称
gamma Synuclein/SNCG overexpression 293T裂解物(whole cell)
参阅全部 gamma Synuclein/SNCG 细胞裂解液 -
常规说明
ab94101 is a 293T cell transfected lysate in which Human gamma Synuclein/SNCG has been transiently over-expressed using a pCMV-gamma Synuclein/SNCG plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. Tissue specificity: Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. Similarity: Belongs to the synuclein family. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94101于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94101 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-gamma Synuclein/SNCG antibody (ab89462) at 1/500 dilution
Lane 1 :gamma Synuclein/SNCG overexpression 293T lysate (whole cell) (ab94101)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94101 尚未被引用在任何文献中。