重组Anti-gamma Synuclein/SNCG抗体[EP1539Y] (ab52633)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1539Y] to gamma Synuclein/SNCG
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-gamma Synuclein/SNCG抗体[EP1539Y]
参阅全部 gamma Synuclein/SNCG 一抗 -
描述
兔单克隆抗体[EP1539Y] to gamma Synuclein/SNCG -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide within gamma Synuclein/SNCG aa 100-200. The exact sequence is proprietary.
-
阳性对照
- WB: HeLa and SK-BR-3 cell lysates; Human fetal brain lysate. IHC-P: Human brain.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1539Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52633于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 13 kDa).
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/1000 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 13 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. -
组织特异性
Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. -
序列相似性
Belongs to the synuclein family. -
翻译后修饰
Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. -
细胞定位
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. - Information by UniProt
-
数据库链接
- Entrez Gene: 6623 Human
- Omim: 602998 Human
- SwissProt: O76070 Human
- Unigene: 349470 Human
-
别名
- BCSG1 antibody
- Breast cancer specific gene 1 protein antibody
- Breast cancer specific protein 1 antibody
see all
图片
-
All lanes : Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SNCG knockout HeLa cell lysate
Lane 3 : SK-BR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 13 kDaLanes 1-3: Merged signal (red and green). Green - ab52633 observed at 16 kDa. Red - loading control ab8245 observed at 36 kDa.
ab52633 Anti-gamma Synuclein/SNCG antibody [EP1539Y] was shown to specifically react with gamma Synuclein/SNCG antibody in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265623 (knockout cell lysate ab257694) was used. Wild-type and gamma Synuclein/SNCG knockout samples were subjected to SDS-PAGE. ab52633 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Anti-gamma Synuclein/SNCG antibody [EP1539Y] (ab52633) at 1/2000 dilution + Human fetal brain lysate
Predicted band size: 13 kDa -
ab52633 staining human brain in IHC-P.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab52633 被引用在 4 文献中.
- Fei J et al. Inhibition of SNCG suppresses the proliferation of lung cancer cells induced by high glucose. Mol Med Rep 23:N/A (2021). PubMed: 33313952
- Chen Z et al. The down-regulation of SNCG inhibits the proliferation and invasiveness of human bladder cancer cell line 5637 and suppresses the expression of MMP-2/9. Int J Clin Exp Pathol 13:1873-1879 (2020). PubMed: 32782717
- Wang Y et al. Hypoxia-inducible lncRNA-AK058003 promotes gastric cancer metastasis by targeting ?-synuclein. Neoplasia 16:1094-106 (2014). WB . PubMed: 25499222
- Kim SH et al. Frequent expression of follicular dendritic cell markers in Hodgkin lymphoma and anaplastic large cell lymphoma. J Clin Pathol 66:589-96 (2013). IHC ; Human . PubMed: 23454725