概述

  • 产品名称Anti-gamma Synuclein抗体
    参阅全部 gamma Synuclein 一抗
  • 描述
    兔多克隆抗体to gamma Synuclein
  • 特异性The immunizing peptide was chosen to avoid cross-reactivity of the antiserum to other synucleins. Immunohistochemical and western blot analysis of human brain indicates a high level of specificity for this antiserum.
  • 经测试应用适用于: ICC/IF, IP, IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide

    EKEEVAEEAQSGGD

    corresponds to amino acids 114-127 of the human gamma synuclein protein. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC) and coupled to diphtheria toxoid.

性能

应用

Our Abpromise guarantee covers the use of ab6169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IP
IHC-P
IHC-Fr
WB
  • 应用说明ICC/IF: 1/100 - 1/150.
    IHC-P: 1/100.
    IHC-Fr: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000.
    1 site ELISA: Not tested.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
    • 组织特异性Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
    • 序列相似性Belongs to the synuclein family.
    • 翻译后修饰Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
    • 细胞定位Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BCSG1 antibody
      • Breast cancer specific gene 1 protein antibody
      • Breast cancer specific protein 1 antibody
      • Breast cancer-specific gene 1 protein antibody
      • Gamma synuclein antibody
      • Gamma-synuclein antibody
      • Persyn antibody
      • PRSN antibody
      • SNCG antibody
      • SR antibody
      • Synoretin antibody
      • Synuclein gamma antibody
      • synuclein, gamma (breast cancer-specific protein 1) antibody
      • SYUG_HUMAN antibody
      see all

    Anti-gamma Synuclein antibody 图像

    • ab6169 was tested on recombinant protein only, 100ng recombinant protein each lane. 1= alpha-synuclein, 2 =  beta-synuclein, 3 = gamma-synuclein. The primary was 1/2000, secondary was alkaline phosphatase conjugated anti-rabbit from Sigma. We did not try it on crude samples such as brain or other tissue homogenates.

    • ICC/IF image of ab6169 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6169, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • IHC image of ab6169 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6169, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-gamma Synuclein antibody (ab6169)参考文献

    This product has been referenced in:
    • Newman AJ  et al. A New Method for Quantitative Immunoblotting of Endogenous a-Synuclein. PLoS One 8:e81314 (2013). WB ; Human . Read more (PubMed: 24278419) »
    • Wersinger C & Sidhu A Partial regulation of serotonin transporter function by gamma-synuclein. Neurosci Lett 453:157-61 (2009). WB, ICC/IF, IP ; Human, Rat . Read more (PubMed: 19429025) »

    See all 7 Publications for this product

    Product Wall

    Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

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    Thank you for your enquiry. The only gamma-synuclein antibody which we currently have is ab6169, which is not directed against mouse. To see if another company may have what you are looking for, I suggest that you click on the link to "The World's Anti...

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    We tested the antibody on recombinant protein only 100ng recombinant protein each lane. The primary was 1/2000, secondary was alkaline phosphatase conjugated anti-rabbit from Sigma. We did not try it on crude samples such as brain or other tissue homog...

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    The antibody should work on paraffin embedded breast cancer tissue, as it works fine with paraffin embedded human brain tissue which contained abundant gamma-synuclein. However, we have not tried any non-neural tissues.

    For neurological tissue, the use of a scientific microwave is preferable, as it can be set to just below boiling to help minimise the risk of section dissociation. We have no experience of this particular antibody in non-neural tissue. For a general...

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    Try using this antibody at the recommended dilution of 1:1000, then adjust the concentration accordingly from the results. Make sure you perform antigen retrieval before staining (I'll send you my protocol). If doing immunofluorescence, use PBS f...

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    If the centriole contains tubulin then this antibody will detect it.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"