- 数据表
- 具体的参考文献 (8)
- 实验方案
概述
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产品名称Anti-gamma Synuclein抗体
参阅全部 gamma Synuclein 一抗 -
描述兔多克隆抗体to gamma Synuclein
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宿主Rabbit
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特异性ab55424 detects endogenous levels of total gamma Synuclein protein.
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经测试应用适用于: IHC-Fr, WB, IHC-P, ELISA, ICC/IFmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide derived from human gamma Synuclein.
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阳性对照
- human lung carcinoma tissue and HT29 cell extract.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab55424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/10000. | |
| ICC/IF | Use at an assay dependent concentration. |
靶标
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功能Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
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组织特异性Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
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序列相似性Belongs to the synuclein family.
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翻译后修饰Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
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细胞定位Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
- Information by UniProt
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数据库链接
- Entrez Gene: 6623 Human
- Entrez Gene: 20618 Mouse
- Entrez Gene: 64347 Rat
- Omim: 602998 Human
- SwissProt: O76070 Human
- SwissProt: Q9Z0F7 Mouse
- SwissProt: Q63544 Rat
- Unigene: 349470 Human
see all -
别名
- BCSG1 antibody
- Breast cancer specific gene 1 protein antibody
- Breast cancer specific protein 1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Synuclein antibody (ab55424)This image is courtesy of an Abreview submitted by Marta Agudo-Barriusoab55424 staining gamma Synclein in Rat retina cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized by freezing and blocked with 2% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in 2% Triton + 2% Serum in PBS) for 8 hours at 4°C. An Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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Immunohistochemistry (Paraffin-embedded sections) - gamma Synuclein antibody (ab55424)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab55424 (1/50 dilution), in the presence or absence of the immunizing peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab55424 (1/50 dilution), in the presence or absence of the immunizing peptide. -
Western blot - gamma Synuclein antibody (ab55424)All lanes : Anti-gamma Synuclein antibody (ab55424) at 1/500 dilution
Lane 1 : HT29 cell extract, without immunizing peptide
Lane 2 : HT29 cell extract, with immunizing peptide
Predicted band size: 13 kDa
Observed band size: 13 kDa -
Immunohistochemistry (Frozen sections) - gamma Synuclein antibody (ab55424)This image is courtesy of an Abreview submitted by Kevin Jinab55424 staining gamma synuclein in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in P) for 12 hours at 4°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody. -
Immunohistochemistry (Frozen sections) - Anti-gamma Synuclein antibody (ab55424)Image courtesy of an anonymous Abreview.ab55424 staining gamma Synuclein in murine retinal tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in formaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab55424 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution. -
Immunohistochemistry (Frozen sections) - Anti-gamma Synuclein antibody (ab55424)Image courtesy of an anonymous Abreview.ab55424 staining gamma Synuclein in rat retinal tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab55424 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.
实验方案
文献
This product has been referenced in:
- Mather Q et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). Read more (PubMed: 28781809) »
- Sayyad Z et al. 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively. Sci Rep 7:16855 (2017). ICC/IF . Read more (PubMed: 29203899) »
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