- 数据表
- 具体的参考文献 (4)
- 实验方案
概述
- 产品名称Anti-gamma Synuclein抗体
参阅全部 gamma Synuclein 一抗 - 描述兔多克隆抗体to gamma Synuclein
- 特异性ab55424 detects endogenous levels of total gamma Synuclein protein.
- 经测试应用适用于: IHC-Fr, WB, IHC-P, ELISA, ICC/IFmore details
- 种属反应性与反应: Mouse, Rat, Human
- 免疫原
Synthetic peptide derived from human gamma Synuclein.
- 阳性对照
- human lung carcinoma tissue and HT29 cell extract.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Immunohistochemistry kits
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab55424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/10000. | |
| ICC/IF | Use at an assay dependent concentration. |
靶标
- 功能Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
- 组织特异性Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
- 序列相似性Belongs to the synuclein family.
- 翻译后修饰Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
- 细胞定位Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
- Information by UniProt
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数据库链接
- Entrez Gene: 6623 Human
- Entrez Gene: 20618 Mouse
- Entrez Gene: 64347 Rat
- Omim: 602998 Human
- SwissProt: O76070 Human
- SwissProt: Q9Z0F7 Mouse
- SwissProt: Q63544 Rat
- Unigene: 349470 Human
see all -
别名
- BCSG1 antibody
- Breast cancer specific gene 1 protein antibody
- Breast cancer specific protein 1 antibody
see all
Anti-gamma Synuclein antibody 图像
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Synuclein antibody (ab55424)This image is courtesy of an Abreview submitted by Marta Agudo-Barriusoab55424 staining gamma Synclein in Rat retina cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized by freezing and blocked with 2% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in 2% Triton + 2% Serum in PBS) for 8 hours at 4°C. An Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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Immunohistochemistry (Paraffin-embedded sections) - gamma Synuclein antibody (ab55424)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab55424 (1/50 dilution), in the presence or absence of the immunizing peptide.Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab55424 (1/50 dilution), in the presence or absence of the immunizing peptide. -
Western blot - gamma Synuclein antibody (ab55424)All lanes : Anti-gamma Synuclein antibody (ab55424) at 1/500 dilution
Lane 1 : HT29 cell extract, without immunizing peptide
Lane 2 : HT29 cell extract, with immunizing peptide
Predicted band size : 13 kDa
Observed band size : 13 kDa -
Immunohistochemistry (Frozen sections) - gamma Synuclein antibody (ab55424)This image is courtesy of an Abreview submitted by Kevin Jinab55424 staining gamma synuclein in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in P) for 12 hours at 4°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody. -
Immunohistochemistry (Frozen sections) - Anti-gamma Synuclein antibody (ab55424)Image courtesy of an anonymous Abreview.ab55424 staining gamma Synuclein in murine retinal tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in formaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab55424 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution. -
Immunohistochemistry (Frozen sections) - Anti-gamma Synuclein antibody (ab55424)Image courtesy of an anonymous Abreview.ab55424 staining gamma Synuclein in rat retinal tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab55424 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.
Anti-gamma Synuclein antibody (ab55424)参考文献
This product has been referenced in:
- Wang L et al. Downregulation of microRNA-497 is associated with upregulation of synuclein ? in patients with osteosarcoma. Exp Ther Med 12:3761-3766 (2016). WB ; Human . Read more (PubMed: 28105108) »
- Surgucheva I et al. Cell-Specific Post-Transcriptional Regulation of ?-Synuclein Gene by Micro-RNAs. PLoS One 8:e73786 (2013). WB . Read more (PubMed: 24040069) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"