Anti-gamma Sarcoglycan抗体(ab104478)


  • 产品名称
    Anti-gamma Sarcoglycan抗体
    参阅全部 gamma Sarcoglycan 一抗
  • 描述
    兔多克隆抗体to gamma Sarcoglycan
  • 宿主
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human protein (NP_000222.1)

  • 阳性对照
    • Human kidney tissue lysate, Transfected 293T cell line IHC-P: FFPE human heart tissue sections.



Our Abpromise guarantee covers the use of ab104478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能
    Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
  • 组织特异性
    Expressed in skeletal and heart muscle.
  • 疾病相关
    Defects in SGCG are the cause of limb-girdle muscular dystrophy type 2C (LGMD2C) [MIM:253700]. LGMD2C is characterized by progressive muscle wasting from early childhood.
  • 序列相似性
    Belongs to the sarcoglycan beta/delta/gamma/zeta family.
  • 细胞定位
    Cell membrane > sarcolemma. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 35 kDa dystrophin associated glycoprotein antibody
    • 35 kDa dystrophin-associated glycoprotein antibody
    • 35DAG antibody
    • 35kD dystrophin associated glycoprotein antibody
    • 35kDa dystrophin-associated glycoprotein antibody
    • A4 antibody
    • DAGA4 antibody
    • DMDA antibody
    • DMDA1 antibody
    • Gamma SG antibody
    • Gamma-sarcoglycan antibody
    • Gamma-SG antibody
    • LGMD2C antibody
    • MAM antibody
    • MGC130048 antibody
    • Sarcoglycan gamma antibody
    • SCARMD2 antibody
    • SCG3 antibody
    • SGCG antibody
    • SGCG_HUMAN antibody
    • TYPE antibody
    see all


  • IHC image of gamma Sarcoglycan staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104478, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-gamma Sarcoglycan antibody (ab104478) at 1 µg/ml + Human kidney tissue lysate at 50 µg

    Predicted band size: 32 kDa

  • All lanes : Anti-gamma Sarcoglycan antibody (ab104478) at 1 µg/ml

    Lane 1 : gamma Sarcoglycan transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 32 kDa


This product has been referenced in:
  • van den Bergen JC  et al. Clinical characterisation of Becker muscular dystrophy patients predicts favourable outcome in exon-skipping therapy. J Neurol Neurosurg Psychiatry N/A:N/A (2013). Human . Read more (PubMed: 23667215) »

See 1 Publication for this product


Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry free floating
Rabbit Tissue sections (Olfactory bulb)
Olfactory bulb

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Verified customer

提交于 Jan 05 2017

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The sarcoglycan protein is located in the cell membrane and expressed in kidney, so the membrane kidney lyaste ab30193 should be suitable for both these sarcoglycan antibodies. However, they may like to consider a ...

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Thank you for your enquiry regarding ab104478 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
After reading through the detailed protocol you kindly...

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