Anti-gamma Catenin抗体[15F11] (ab12083)


  • 产品名称Anti-gamma Catenin抗体[15F11]
    参阅全部 gamma Catenin 一抗
  • 描述
    小鼠单克隆抗体[15F11] to gamma Catenin
  • 特异性Ab12083 reacts with plakoglobin.
  • 经测试应用适用于: ICC/IF, Flow Cyt, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    This information is considered to be commercially sensitive



Our Abpromise guarantee covers the use of ab12083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/10. Cells fixed in paraformaldehyde.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/10. Predicted molecular weight: 81.5 kDa.
IP Use at an assay dependent concentration.


  • 功能Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • 疾病相关Defects in JUP are the cause of Naxos disease (NXD) [MIM:601214]. NXD is an autosomal recessive disorder combining diffuse non-epidermolytic palmoplantar keratoderma with arrhythmogenic right ventricular dysplasia/cardiomyopathy and woolly hair.
    Defects in JUP are the cause of familial arrhythmogenic right ventricular dysplasia type 12 (ARVD12) [MIM:611528]; also called arrhythmogenic right ventricular cardiomyopathy 12 (ARVC12). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
  • 序列相似性Belongs to the beta-catenin family.
    Contains 9 ARM repeats.
  • 细胞定位Cell junction > adherens junction. Cell junction > desmosome. Cytoplasm > cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARVD 12 antibody
    • ARVD12 antibody
    • Catenin (cadherin associated protein), gamma 80kDa antibody
    • catenin (cadherin-associated protein) gamma (80kD) antibody
    • Catenin gamma antibody
    • CTNNG antibody
    • Desmoplakin 3 antibody
    • Desmoplakin III antibody
    • Desmoplakin-3 antibody
    • Desmoplakin3 antibody
    • DesmoplakinIII antibody
    • DP 3 antibody
    • DP III antibody
    • DP3 antibody
    • DPIII antibody
    • gamma catenin antibody
    • Junction plakoglobin antibody
    • JUP antibody
    • OTTHUMP00000164732 antibody
    • OTTHUMP00000164735 antibody
    • OTTHUMP00000164738 antibody
    • PDGB antibody
    • PKGB antibody
    • PLAK_HUMAN antibody
    • PLAKOGLOBIN antibody
    see all

Anti-gamma Catenin antibody [15F11] 图像

  • ICC/IF image of ab12083 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12083, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

  • Overlay histogram showing HeLa cells stained with ab12083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12083, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-gamma Catenin antibody [15F11] (ab12083)参考文献

This product has been referenced in:
  • Sacco PA  et al. Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem 270:20201-6 (1995). WB ; Human . Read more (PubMed: 7650039) »
  • Johnson KR  et al. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 207:252-60 (1993). Read more (PubMed: 8344378) »

See all 2 Publications for this product

Product Wall

The exact epitope has not yet been defined for this clone to our knowledge. This clone has been characterised in the publication Sacco. P.A. et al., J. Biol. Chem., 270, 20201-20206 (1995). "Identification of plakoglobin domains required for associatio...

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Thank you for your enquiry. For the generation of the antibody a fusion protein was used. The antibody has been generated as described in the following article: Exp Cell Res. 1993 Aug;207(2):252-60. P- and E-cadherin are in separate complexes in cel...

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Thank you for your patience. Regarding ab12083, our source has informed that they do not have any information about the epitope recognized by clone 15F11. If you have any additional questions, please contact us again.

Thank you for your phone call. Regarding ab15004, our source for this antibody has said that "The epitope is identified only to the extent that it is in the Band 5 protein of Plakoglobin. No further steps for epitope mapping have been performed." On...

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