The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IHC-Fr: 1/200 - 1/2000. depending on tissue and detection method.
IHC-P: 1/200 - 1/2000. depending on tissue and detection method.
IP: Use at an assay dependent dilution.
WB: 1/1000 - 1/4000. depending on detection method. Predicted molecular weight: 91 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
序列相似性Belongs to the adaptor complexes large subunit family. Contains 1 GAE domain.
细胞定位Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Yi L et al. HIV-1 Nef binds a subpopulation of MHC-I throughout its trafficking itinerary and down-regulates MHC-I by perturbing both anterograde and retrograde trafficking. J Biol Chem285:30884-905 (2010).
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