Anti-gamma Actin抗体[2A3] (ab123034)

概述

  • 产品名称
    Anti-gamma Actin抗体[2A3]
    参阅全部 gamma Actin 一抗
  • 描述
    小鼠单克隆抗体[2A3] to gamma Actin
  • 经测试应用
    适用于: IHC-P, WB, ELISA, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Human, Pig, Zebrafish
    预测可用于: a wide range of other species
  • 免疫原

    Synthetic peptide (Ac-

    EEEIAALVI

    -COOH) with an acetylated N-terminus and conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 2-10 of Human gamma Actin

  • 阳性对照
    • Human dermal fibroblast cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    2A3
  • 骨髓瘤
    NS0
  • 同种型
    IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab123034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/100 - 1/500.
IHC-Fr 1/100 - 1/500.
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

靶标

  • 功能
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 疾病相关
    Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 序列相似性
    Belongs to the actin family.
  • 翻译后修饰
    The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog.
  • 细胞定位
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACT antibody
    • ACTB antibody
    • ACTG antibody
    • ACTG_HUMAN antibody
    • actg1 antibody
    • Actin antibody
    • Actin, cytoplasmic 2 antibody
    • Actin, gamma 1 antibody
    • Actin, gamma 1 propeptide antibody
    • Actin, gamma antibody
    • BRWS2 antibody
    • cytoplasmic 2 antibody
    • Cytoskeletal gamma actin antibody
    • Deafness, autosomal dominant 20 antibody
    • Deafness, autosomal dominant 26 antibody
    • DFNA20 antibody
    • DFNA26 antibody
    • epididymis luminal protein 176 antibody
    • Gamma-actin antibody
    • HEL-176 antibody
    • N-terminally processed antibody
    see all

Anti-gamma Actin antibody [2A3] 图像

  • Immunohistochemical analysis of paraffin embedded Human small intestine tissue labelled with Anti-gamma Actin antibody [2A3] at 5 μg/ml

  • ab123034 at 1/500 dilution staining gamma Actin in methanol-fixed Human dermal fibroblasts by Immunofluorescence.

Anti-gamma Actin antibody [2A3] (ab123034)参考文献

ab123034 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hippocampus)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
1 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 27 2017

Yes , this antibody does not react with other isoforms and it binds to actin gamma 2 only.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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