Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT29)
Permeabilization
Yes - 0.2% TritonX100 in PBS
Specification
HT29
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 555 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
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提交于 Nov 07 2012